DEV034967 Supplementary Material

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• Supplemental Figure S1 -

  Fig. S1. Defective neurogenesis, but not morphogenesis, in Fst^−/− olfactory epithelium. Fst^+/− mice (Matzuk et al., 1995) were maintained as described previously (Wu et al., 2003), and crossed to obtain Fst^−/− embryos and wild-type littermates at E17.5. Horizontal cryosections through the heads of Fst^−/− and wild-type littermates were processed for in situ hybridization as described (Wu et al., 2003) with cRNA probes to Ngn1 and Ncam. Anterior is to the right of each panel; posterior, left; lateral, top; and the nasal septum, bottom. In the wild-type nasal cavity, both the highly convoluted posterior turbinate structures (T) and the flat nasal septum (sep) can be seen to be covered by a thick olfactory epithelium (OE) that contains a basal layer of Ngn1-expressing immediate neuronal precursors (INPs) and several layers of Ncam-expressing postmitotic olfactory receptor neurons (ORNs). In Fst^−/− mice at this age (right before birth), the basic size and shape of the nasal cavity are the same as in wild type, and convoluted turbinate structures covered by OE can be easily visualized in the mutants. The OE of Fst^−/− mice is, however, much thinner than that of wild-type littermates (∼40% thinner), as observed previously (Wu et al., 2003), and is relatively devoid of Ngn1-expressing INPs, with a much thinner Ncam-expressing ORN layer (see insets). NC, nasal cavity; T, turbinate; OE, olfactory epithelium; sep, septum. Scale bar: 200 µm.

  Reference

  Matzuk, M. M., Lu, N., Vogel, H., Sellheyer, K., Roop, D. R. and Bradley, A. (1995). Multiple defects and perinatal death in mice deficient in follistatin. Nature 374, 360-363.