DEV004325 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. RNA in situ hybridization with a probe for the floxed sequence of Bmpr1a. (A) At the 10 somite stage (∼E8.75), anterior to the optic cup (oc), expression of Bmpr1a is consistently weaker in the mutant (between arrowheads). (B) At E9.5 in the telencephalic neuroepithelium (ne), expression is undetectable in the mutant (asterisk; silver grains are equal to background levels). me, head mesenchyme. (C) Expression at E9.5 in all layers of the first branchial arch (ba) and in the right atrial chamber of the heart (ac) is preserved in the mutant. (D) Expression at E9.5 in the foregut (fg) is also maintained in the mutant.

• Supplemental Figure S2 -

  Fig. S2. Rfx4-v3 probe on E9.5 coronal sections. Expression is weak, but strongest in the cortical anlage and is similar in the mutant.

• Supplemental Figure S3 -

  Fig. S3. Actr1 expression. Although Actr1 is expressed in differentiating cells at E14.5, its expression is not detected at E9.5.

• Supplemental Figure S4 -

  Fig. S4. Msx1 expression. Msx1 expression at E9.5 in the dorsal midline is lost in the mutant (arrows).