Supplemental Figure S1
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Fig. S1. Interactions among NgCAM, contactin 4 and APPsα in RGC axon extension. (A-C) Analysis of the same experiments as presented in Fig. 7D-F, quantitating axon extension instead of number. A value was assigned to each explant by averaging the ten longest distances (or all if there were fewer than ten axons) from explant to axon tip. *, P<0.005 by unpaired Student’s t-test. #, P<0.05 by paired Student's t-test, using the six matched control explants adjacent to those used for AP-APP(18-205) samples. Error bars, s.e.m. (D) Co-transfection of contactin 4-myc-His and contactin 5-Fc with either control or contactin 4 shRNA virus construct, followed by anti-Fc (red), anti-myc (green) western blot. Quantitation with Li-COR Odyssey scanner is shown beneath the blot. Expression of contactin 4-myc-His was significantly decreased (**, P<0.0001) in the presence of the contactin 4 shRNA construct, whereas expression of contactin 5-Fc was unaffected.
