DEV008920 Supplementary Material

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• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. Br expression in mosaic egg chambers with clones of tkv⁻ cells. Anterior clones of tkv generate ectopic Br expression in the anterior cells. tkv⁻ cells are marked by the absence of GFP (A,D). Br expression is shown in red (B,E).

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Effect of tkv-RNAi on follicle cell patterning and eggshell morphology. (A-C) Expression of tkv-RNAi induced ectopic expression of br transcript and Br protein in the anterior follicle cells and increased the duration of br expression in the roof cells. (D) These changes in the pattern of Br expression led to a predictable change in the expression of rho. (E) In addition, eggshells from tkv− RNAi flies had clear dorsal appendage defects. A large fraction of the eggs (∼30%) had flat dorsal appendages, similar to the ones derived from the egg chambers with ubiquitous expression of Dad (compare with the image in Fig. 5F). (F) A significant number of the eggshells had small extra appendages, presumably reflecting the presence of ectopic br in the dorsal-anterior follicle cells (A).

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. tkv expression in response to ectopic expression of the Br-Z1 isoform. (A) Kinetics of the CY2-Gal4 driver expression reproduced from Fig. C3 in L. A. Goentoro, PhD thesis, Princeton University, 2006; for details of the driver quantification procedure see Goentoro et al. (Goentoro et al., 2006b). (B,C) tkv expression in stage 10A egg chambers. Note that at this stage, the wild-type pattern has two dorsolateral patches. (D) tkv expression in a stage 11 egg chamber.