DEV009654 Supplementary Material

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• Supplemental Figure S1 - Fig. S1. Subcellular localization of PIN1-GFP. (A-D) Localization of PIN1-GFP (green, left) and mRFP or Venus-tagged subcellular marker genes (red, middle). PIN1-GFP and mRFP- or Venus-subcellular markers were co-introduced under the control of their own promoter and the CaMV 35S promoter, respectively, into Arabidopsis protoplasts. Merged image (right) of PIN1-GFP and the late endosome marker ARA6-mRFP (A), the early endosome marker mRFP-ARA7 (B), the cis-Golgi marker Venus-SYP31 (C) and the trans-Golgi-network (TGN) marker Venus-SYP41 (D). Scale bars: 10 μm.

• Supplemental Figure S2 -

  Fig. S2. Subcellular localization of GFP-PID. (A-D) Localization of GFP-PID (green, left) and mRFP or Venus-tagged subcellular marker genes (red, middle). GFP-PID and mRFP- or Venus-subcellular markers were co-introduced under the control of CaMV 35S promoter into Arabidopsis protoplasts. Merged image (right) of GFP-PID and the late endosome marker ARA6-mRFP (A), the early endosome marker mRFP-ARA7 (B), the cis-Golgi marker Venus-SYP31 (C) and the trans-Golgi-network (TGN) marker Venus-SYP41 (D). Scale bars: 10 μm.

• Supplemental Figure S3 -

  Fig. S3. Spatial relationship between PIN1 and PID. PIN1-GFP was expressed under the control of its own promoter in Arabidopsis protoplasts (left). mRFP-PID was expressed under the control of the CaMV 35S promoter in Arabidopsis protoplasts (middle). Merged image (right) of PIN1-GFP and mRFP-PID. Scale bars: 10 μm.