DEV009662 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Distribution of shaven baby transcripts in different tsh Hox mutations. (A) WT ventral, (B) WT lateral, (C) tsh^- HoxCP, (D) tsh^- Hox^- Scr⁺, (E) tsh^- Hox^-Antp⁺, (F) tsh^- Hox^- Ubx⁺, (G) tsh^- Hox^- abdA⁺, (H) tsh^- Hox^- AbdB⁺. In A-C Elav, labelling the central nervous system highlights the ventral part of the embryo. Tsh (blue) marks the thorax and abdomen. The expression of the active Hox proteins is shown in red. svb is detected ventrally in the domains where Hox genes are active in A,B,E-G, whereas in C,D,H, svb transcripts are not detected ventrally. svb is detected dorsally in all genotypes. All embryos are at the retracted germ band stage, with the anterior towards the left.

• Supplemental Figure S2 -

  Fig. S2. Expression of optix (green) in wild type (top) and HoxCP mutant (bottom) embryos. Elav (red) marks the nervous system..