DEV010447 Supplementary Material

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  Fig. S1. Cell movements are defective in egg chambers lacking core planar polarity gene function. (A) Chart showing the extent of border cell migration relative to outer follicle cell rearrangement at stage 9 in different mutant backgrounds. Loss-of-function alleles of core planar polarity genes were crossed out to a w¹¹¹⁸ ‘wild-type’ strain for several generations to provide a common genetic background. Border cell migration was scored relative to rearrangement of the outer follicle cells (the scoring scheme is as described in legend to Fig. 1). Both a medium-strength (fz²⁵) and strong (fz²¹) allele of fz show a significant increase in slower migrating clusters (‘behind’). Similarly, the dsh¹ planar polarity-specific allele of dsh, and the stbm⁶ and pk^pk-sple-13 null alleles, also show significant delays in border cell migration. As controls, we examined the effect on border cell migration of loss of Wnt4 (using the heteroallelic combination of Wnt4^C1/Wnt4^EMS23) or slow border cells (slbo). Wnt4 has previously been reported to affect earlier stages of ovary morphogenesis via the frizzled homologue Dfrizzled2, but does not affect planar polarity signalling (Cohen et al., 2002). Consistent with this, border cell migration is not delayed in this genotype. Conversely, slbo function is absolutely required for border cell migration (Montell et al., 1992) and, in this genotype, all clusters remain in an anterior position. (B) Chart showing the proportion of border cell clusters that have completed posterior migration by stage 10 in egg chambers from w¹¹¹⁸ (n=595), fz²¹ (n=350) or stbm⁶ (n=301) mutant individuals. Thus, planar polarity gene function is not absolutely required for the completion of posterior border cell migration.

  References

  Cohen, E. D., Mariol, M.-C., Wallace, R. M. H., Weyers, J., Kamberov, Y. G., Pradel, J. and Wilder, E. L. (2002). Dwnt4 regulates cell movement and focal adhesion kinase during Drosophila ovarian morphogenesis. Dev. Cell 2, 437-448.