DEV013540 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. MEFs that lack Dvl1 and Dvl2 (Dvl1^{−/−; Dvl2−/−).} MEF cell extracts were immunoblotted with the antibodies indicated. WT, wild-type MEFs.

• Supplemental Figure S2 -

  Fig. S2. Knockdown of Axin2 expression by additional shRNAs reduces Wnt-induced Lrp6 phosphorylation in Axin^−/− ES cells. Axin^−/− ES cells were infected with three independent lentiviral shRNAs against mouse Axin2 and then treated with Wnt3a CM or control CM. The shRNA-infected cells show reduced Lrp6 (endogenous) phosphorylation compared with the control ES cells.