DEV014894 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Mineral oil prevents PI3K inhibition by LY294002 and wortmannin. (A) PtdIns(3,4,5)P₃ labeling in two-cell embryos (left and right) and a 16-cell morula (middle) after overnight culture in the presence of 100 nM wortmannin (left and middle) or 100 µM LY294002 (right), under mineral oil. Note that the PI3K inhibitors failed to prevent PtdIns(3,4,5)P₃ accumulation. (B) Inhibition of PtdIns(3,4,5)P₃ synthesis in two-cell embryos (left and right) and a 16-cell morula (middle) after a one-hour incubation with 100 nM wortmannin (left and middle) or 10 µM LY294002 (right), in the absence of mineral oil. Confocal images are representative of at least eight similar observations.