DEV015156 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-S3 (Adobe PDF) - Adobe PDF

• Supplemental Figure S1 -

  Fig S1. Seminal receptacle in Hr39 mutants continues to store sperm. Confocal microscopy images of wild-type (A) and Hr39⁰³⁵⁰⁸ mutant (B) seminal receptacle stained with DAPI (DNA). Arrows indicate sperm heads within coiled seminal receptacle. Scale bar: 50 µm.

• Supplemental Figure S2 -

  Fig S2. Analysis of Hr39 mutants over deficiency, Df(2L)Exel6048. Percentage of transheterozygote females (Hr39/Df(2L)Exel6048) of each insertion allele possessing 0, 1, 2 or 3 spermathecae. All the Hr39 alleles fail to complement Df(2L)Exel6048, but produce on average a more normal complement of spermathecae than the corresponding homozygotes (compare Fig. S2 with Fig. 1H). This is consistent with molecular data showing that the Hr39 locus is not fully deleted by Exel6048 (see Table S1 in the supplementary material), but retains the entire 5′ upstream region as well as nucleotides +1 to +34 of exon 1. Such partial complementation between upstream sequences and insertion alleles has been observed frequently and is termed ‘transvection’.

• Supplemental Figure S3 -

  Fig S3. Normal patterning of the female genital disc in Hr39 mutants. Confocal microscopy images of female genital discs from wild type and homozygous Hr39^ly92 and Hr39⁰⁴⁴⁴³ mutants. Anterior of the discs is upwards in each panel and all panels are projections with DNA in magenta. (A-C) Expression pattern of dachshund (dac) in the female genital disc detected by an anti-DAC antibody (green). Overlap is in white. (A) Wild type female, (B) Hr39^ly92 female and (C) Hr39⁰⁴⁴⁴³ female. (D,E) Expression pattern of wingless (wg) in the female genital disc detected by an anti-WG antibody (green). Overlap is in white. (D) Wild-type female, (E) Hr39^ly92 female and (F) Hr39⁰⁴⁴⁴³ female.