DEV015982 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Test of Src42A RNAi construct. A hairpin RNAi construct of Src42A was expressed by ptc-Gal4. It strongly reduced the phospho-Src signal (red) at the compartment boundary (green GFP signal).

• Supplemental Figure S2 -

  Fig. S2. Src mutations do not alter Spalt (Sal) expression in the trachea. Embryos with btl>GFP-moesin (green) were labeled with the dorsal trunk marker anti-Spalt. (A) control, (B) src42A^myri; src64B^P1, (C) btl>src42A^ACT.

• Supplemental Figure S3 -

  Fig. S3. Synergistic effect of Src42A^ACT and TCFΔN in downregulation of E-cadherin. (A-D) Src42A^ACT and TCFΔN were expressed either singly or doubly in btl>GFP-moesin (green) embryos. E-cadherin staining is shown in grayscale. Compare the level of E-cadherin in the trachea (arrowhead) with that in dorsal epidermis (arrow). In B, enlargement of a part of trachea with reduced E-cadherin levels (red arrowhead) is shown. In D, co-expression of Src42A^ACT and TCFΔN severely disrupted tracheal epithelium and reduced the E-cadherin level.

• Movie 1 -

  Movie 1. Time-lapse movie of dorsal branch elongation in a control embryo (see Fig. 2A). AJs in the trachea were labeled with btl>α-catenin-GFP.

• Movie 2 -

  Movie 2. A src42A^myri embryo (see Fig. 2B).

• Movie 3 -

  Movie 3. A btl>src42A^RNAi; src42A^26-1/+ embryo (see Fig. 2C).

• Movie 4 -

  Movie 4. A src42A^myri; src64B^P1 embryo (see Fig. 2D).

• Movie 5 -

  Movie 5. Decay of AJs in dorsal branches of a shg^IH/shg^E17B mutant with btl>α-catenin-GFP (see Fig. 3C).

• Movie 6 -

  Movie 6. Dorsal branches of btl>GFP-moesin embryos. Control (left) and btl>src42A^ACT (right; see Fig. 3A,E).

• Movie 7 -

  Movie 7. Dorsal branches of btl>E-cadherin-GFP embryos. Control (left) and btl>src42A^ACT (right; see Fig. 3D,H).

• Movie 8 -

  Movie 8. Metastatic behavior of tracheal cells expressing Src42A^ACT (see Fig. 7A).

• Movie 9 -

  Movie 9. Embryos with btl>E-cadherin-GFP embryos. Control (top), btl>src42A^ACT (middle) and btl>src42A^ACT + TcdDN (bottom; see Fig. 7B,D,F).