DEV017947 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1-6 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Phenotypes associated with CPC-homolog loss-of-function mutants. (A) Number of root hairs per mm was determined by counting a minimum of ten 5-day-old seedlings from each line. Data are mean±s.d. (B) Number of trichomes per leaf was determined by counting a minimum of ten 2-week-old third leaves from each line. Data are mean±s.d. Asterisks indicate too numerous to count.

• Supplemental Figure S2 -

  Fig. S2. Trichome phenotype of the cpl3 cpc try triple mutant. Trichome clustering on 2-week-old third leaves of the (A) cpc try double mutant and (B) cpl3 cpc try triple mutant. Scale bars: 1 mm.

• Supplemental Figure S3 -

  Fig. S3. Phenotypes of the cpl3 cpc try etc1 quadruple mutant. (A) Hypocotyl of a cpl3 cpc try etc1 quadruple mutant was covered by trichomes. (B) DIC image of the quadruple mutant. Scale bars: 100 µm in B; 200 µm in A.

• Supplemental Figure S4 -

  Fig. S4. Trichome phenotypes of soil-grown plants. (A) First leaves of Col-0 and cpl3 mutant in 10-day-old soil-grown plants. (B) Trichomes of Col-0 and cpl3 mutant in 3-week-old soil-grown plant leaves. Scale bars: 100 µm in B; 1 mm in A.

• Supplemental Figure S5 -

  Fig. S5. Phenotypes associated with CPL3 gain-of-function plants. (A) Number of root hairs per mm was determined by counting a minimum of ten 5-day-old seedlings from each line. Data are mean±s.d. (B) Number of trichomes per leaf was determined by counting a minimum of ten 2-week-old third leaves from each line. Data are mean±s.d. (C,D) Semi-quantitative RT-PCR analysis of CPL3 transcripts. Transgenic seedlings of CPL3p::CPL3 (C) and 35S::CPL3 (D) were grown for 5 days. Total RNA was prepared from the seedlings and subjected to RT-PCR. 35 cycles were used for CPL3 and 28 cycles for the EFα control.

• Supplemental Figure S6 -

  Fig. S6. Protein interactions with CPC-like MYB proteins (CPC, TRY, ETC1, ETC2 and CPL3) versus bHLH (GL3, EGL3 and AtMYC1) using a yeast two-hybrid assay. Comparison of interactions between (A) CPC-like MYB and GL3, (B) CPC-like MYB and EGL3 and (C) CPC-like MYB and MYC1. The activity of β-galactosidase was assayed using three to six independently transformed yeast lines. Yeast containing plasmids without fused proteins (BD) were used as a negative control. AD, GAL4 transcriptional activation domain; BD, GAL4 DNA-binding domain.

• Supplemental Figure S7 -

  Fig. S7. Expression of CPCp::GUS, TRYp::GUS, ETC1p::GUS, ETC2p::GUS and CPL3p::GUS. Expression was assessed in 7-day-old roots (A-E) and leaves (F-J), and in 2-week-old leaves (K-O). Scale bars: 100 µm in A; 1 mm in F,K.

• Supplemental Figure S8 -

  Fig. S8. Expression of CL3Cp::GUS in Col-0, cpc, try, etc1, etc2 and cpl3 mutant 2-week-old leaves.