DEV022707 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 (Adobe PDF) -

  Table S1. Frequency of viable Sox2Cre;c-myc^flox/flox embryos during embryonic development.

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. CD34 is expressed in control and mutant CD45+Kit+AA4.1+ stem/progenitors.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Normal development of the pancreas and the primordial germ cells (PGCs) in c-Myc-deficient embryos. (A) Pdx1 expression reveals the pancreas anlage at E10.5. (B) Quantification of primoridial germ cells revealed by staining for alkaline phosphatase at E9.5. No difference was detected in the localization and the total number of PGCs in control compared with Sox2Cre;c-myc^flox/flox embryos.

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. Normal liver development in the absence of c-Myc. Histological analysis (H&E) of control and Hnf4aCre;c-myc^flox/flox fetal livers between E11.5-E16.5.

• Supplemental Figure S4 (Adobe PDF) -

  Fig. S4. Real time RT-PCR expression of hematopoietic regulators. CD45⁺ hematopoietic cells of control and mutant embryos were compared for the expression of known regulators of embryonic hematopoiesis. Fold changes in mRNA expression of Scl, Runx1, Gata1, Gata2, Klf4, Klf6 and p21^CIP1 are shown. Significant upregulation of the c-Myc target genes p21 (2.1 -fold) and Klf4 (1,6-fold) is observed. Gene expression levels of Scl, Runx1, Gata1, Gata2 and Klf6 are unchanged in mutant CD45⁺ cells compared with controls.