DEV025809 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Control of abd-A and Abd-B expression by their own products. (A,B) Female genital discs of UAS-abd-A/tub-Gal80^ts; dpp-Gal4 UAS-GFP/MKRS, abd-A-lacZ larvae, kept at 29°C for 3 days during the second and early third larval periods, showing that high levels of Abd-A in the dpp-domain (marked by GFP expression, in green in A) does not eliminate abd-A-lacZ expression (A, B, in blue). (C,D) In UAS-Abd-B(m)/+; dpp-Gal4 UAS-GFP/Abd-B-lacZ genital discs, the Abd-B-lacZ expression (red) is reduced by the increased expression of Abd-B in the dpp domain (marked by GFP expression, in green in C).

• Supplemental Figure S2 -

  Fig. S2. Transient Ubx expression in the posterior compartment of the embryonic wing disc. (A-D) In the posterior compartments of the early leg and wing discs of UAS-GFP/+; hh-Gal4/ Dll-304-lacZ stage 12 embryos, marked by GFP (green, A, marking P compartments) and anti-β galactosidase antibody (red, B, marking the leg, haltere and wing primordia; outlined in C), there is Ubx expression, detected with an anti-Ubx antibody (blue in C). Merged image in D. (E-H). In stage 16 UASGFP/esg-lacZ; hh-Gal4/+ embryos, by contrast, there is no Ubx expression in the wing disc. esg-lacZ (red in F) marks the wing and haltere primordia (outlined in G). Posterior compartments and Ubx expression marked as in A-D. Merged image in H. T2 and T3 indicates the second and third thoracic segments, respectively.