DEV02669 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Summary of results from all constructs used in this study. Maps of constructs are shown on the left. The number of independent insertions analyzed, the effects on the expression pattern in eye and ocelli, as well as relative levels of expression are summarized on the right. A few lines that displayed apparent insertion-site-dependent effects were not considered in this analysis and are not listed.

• Supplemental Figure 2 -

  Fig. S2. Sequence alignment of the conserved IC1, IC2, A1 and A2 boxes. The binding sites for So, Pax6, Mad and Su(H) are highlighted.

• Supplemental Figure 3 -

  Fig. S3. A1 or A2 cannot drive expression in the eye. The 348 bp region containing both A1 and A2 conserved boxes drives reporter gene expression in the eye, but each conserved region alone is not sufficient. Expression was detected by staining for β-gal activity.

• Supplemental Figure 4 -

  Fig. S4. EMSA with Toy protein. The position of the shifted Toy-III complex is marked by an arrow. Toy shifts probe III (lane 4) but not the other probes (lanes 2,3,5,6,7). The binding of Toy to probe III can be competed by unlabeled probe III DNA (lane 10) but not by unlabeled Pax6^MUT-III DNA (lane 11). Probes (I-VI), protein (Toy) and/or competitor DNA (S,NS) added to each reaction are listed above each lane. Reticulocyte lysate (L) only was added in the negative controls (lanes 1,8). The lower band (arrowhead) reflects a non-specific shift due to the lysate. S, specific: unlabeled probe III DNA. NS, non-specific: unlabeled probe III with the Pax6^MUT site.