DEV02753 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Determination of a synchronized population of seedlings. (A) Germination and seedling growth at different time points in a 7.5-hour time course. The stage where the radicle appears is time 0 hours. Time lapse recordings were made of vertically oriented seedlings with a CCD camera connected to a PC fitted with a Scion LC3 framegrabber board and ScionImage software. Arrowheads indicate root tip and shoot apical meristem. (B) Average seedling length (root + hypocotyl) for five seedlings. Error bars, s.e.m.

• Supplemental Figure 2 -

  Fig. S2. Role of targeted AUX1 expression in lateral root formation. Lateral root densities in wild-type (Col-0), aux1-22, parental lines (UAS:AUX1,aux1-22; J1701,aux1-22; M0013,aux1-22 and Q1120,aux1-22), and the line where J1701, M0013, or Q1220 drives AUX1 expression in aux1-22 (J1701>>AUX1,aux1-22; M0013>>AUX1,aux1-22; and Q1220>>AUX1,aux1-22) for seedlings grown at 90°F (A) or 45°F (B)**(P<0.001) and *(P<0.05) above the bars show statistically significant difference for values compared to wild type, as determined by the Student’s t-test. Error bars, s.e.m.