DEV02766 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Hts antibody localization in hts truncation mutants. Localization of designated Hts antibodies in w¹¹¹⁸ (A-D), hts^ΔG (E-H) and hts^W532X (I-L) ovaries. (E,F) In hts^ΔG htsF and 1B1 localize to germline cytoplasm, and follicle cell membranes and cytoplasm. (G,H) In hts^ΔG htsM and htsRC do not show any staining above background. (I-L) In hts^W532X no antibody shows staining above background. (M) Western blot of ovary extracts probed with htsF. The epitope for this protein should be present in both truncations; however, the truncation product is only in hts^ΔG, and not in hts^W532X. Scale bars, 40 μm.

• Supplemental Figure 2 -

  Fig. S2. hts^ΔG mutants lack fusome staining and have abnormal accumulation of Hts protein in the cytoplasm. (A) w¹¹¹⁸ and (B) hts^ΔG germaria stained with 1B1 antibody. The hts^ΔG germarium has no fusome structure and shows cytoplasmic staining in the germline cells that is not seen in w¹¹¹⁸ . (C) w¹¹¹⁸ and (D) hts^ΔG mid-stage egg chambers stained with 1B1 antibody. hts^ΔG shows accumulation of Hts protein aberrantly in the cytoplasm of the germline cells. Scale bars, 10 μm.