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Development 129, 4719-4728 (2002)
Copyright © 2002 The Company of Biologists Limited

The migration of cerebellar rhombic lip derivatives

Jonathan D. Gilthorpe1, Elli-Kalliopi Papantoniou1, Alain Chédotal2, Andrew Lumsden1 and Richard J. T. Wingate1,*

1 MRC Centre for Developmental Neurobiology, King’s College London, New Hunt’s House, Guy’s Campus, London SE1 1UL, UK
2 INSERM U106, Bâtiment de Pédiatrie, Hôpital de la Salpétrière, 47 boulevard de l’ Hôpital, F-75651 Paris cedex 13, France

*Author for correspondence (e-mail: richard.wingate{at}kcl.ac.uk)

Accepted 12 June 2002

We have used cell labelling, co-culture and time-lapse confocal microscopy to investigate tangential neuronal migration from the rhombic lip. Cerebellar rhombic lip derivatives demonstrate a temporal organisation with respect to their morphology and response to migration cues. Early born cells, which migrate into ventral rhombomere 1, have a single long leading process that turns at the midline and becomes an axon. Later born granule cell precursors also migrate ventrally but halt at the lateral edge of the cerebellum, correlating with a loss of sensitivity to netrin 1 and expression of Robo2. The rhombic lip and ventral midline express Slit2 and both early and late migrants are repelled by sources of Slit2 in co-culture. These studies reveal an intimate relationship between birthdate, response to migration cues and neuronal fate in an identified population of migratory cells. The use of axons in navigating cell movement suggests that tangential migration is an elaboration of the normal process of axon extension.

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Key words: Chick, netrin, slit, Robo, GFP, Time-lapse confocal microscopy






© The Company of Biologists Ltd 2002