spacer gif spacer gif spacer gif spacer gif Online submission spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

doi: 10.1242/10.1242/dev.00199

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, W.-M.
Right arrow Articles by Ruohola-Baker, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, W.-M.
Right arrow Articles by Ruohola-Baker, H.
Development 130, 173-184 (2003)
Copyright © 2003 The Company of Biologists Limited

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila

Wu-Min Deng1,*, Martina Schneider2,*, Richard Frock1, Casimiro Castillejo-Lopez2,{dagger}, Emily Anne Gaman1, Stefan Baumgartner2 and Hannele Ruohola-Baker1,{ddagger}

1 Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA
2 Department of Cell and Molecular Biology, Section of Developmental Biology, Lund University, Sweden
{dagger} Present address: EBC, Jämförande Fysiologi (Comparative Physiology) Norbyv. 18A, 752 36 Uppsala, Sweden

{ddagger} Author for correspondence (e-mail: hannele{at}u.washington.edu)

Accepted 11 October 2002

The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.

Key words: Polarity, Axis, Asymmetry, Oogenesis, Epithelia, Dystroglycan, Actin, Microtubule, ECM, Planar polarity, Signaling, Drosophila




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Y. P. Wairkar, L. G. Fradkin, J. N. Noordermeer, and A. DiAntonio
Synaptic Defects in a Drosophila Model of Congenital Muscular Dystrophy
J. Neurosci., April 2, 2008; 28(14): 3781 - 3789.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Akhavan, S. N. Crivelli, M. Singh, V. R. Lingappa, and J. L. Muschler
SEA domain proteolysis determines the functional composition of dystroglycan
FASEB J, February 1, 2008; 22(2): 612 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. J. Allikian, G. Bhabha, P. Dospoy, A. Heydemann, P. Ryder, J. U. Earley, M. J. Wolf, H. A. Rockman, and E. M. McNally
Reduced life span with heart and muscle dysfunction in Drosophila sarcoglycan mutants
Hum. Mol. Genet., December 1, 2007; 16(23): 2933 - 2943.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Haines, S. Seabrooke, and B. A. Stewart
Dystroglycan and Protein O-Mannosyltransferases 1 and 2 Are Required to Maintain Integrity of Drosophila Larval Muscles
Mol. Biol. Cell, December 1, 2007; 18(12): 4721 - 4730.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. Yatsenko, E. E. Gray, H. R. Shcherbata, L. B. Patterson, V. D. Sood, M. M. Kucherenko, D. Baker, and H. Ruohola-Baker
A Putative Src Homology 3 Domain Binding Motif but Not the C-terminal Dystrophin WW Domain Binding Motif Is Required for Dystroglycan Function in Cellular Polarity in Drosophila
J. Biol. Chem., May 18, 2007; 282(20): 15159 - 15169.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
V. Mirouse, L. L. Swick, N. Kazgan, D. St Johnston, and J. E. Brenman
LKB1 and AMPK maintain epithelial cell polarity under energetic stress
J. Cell Biol., May 7, 2007; 177(3): 387 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Hnia, G. Hugon, F. Rivier, A. Masmoudi, J. Mercier, and D. Mornet
Modulation of p38 Mitogen-Activated Protein Kinase Cascade and Metalloproteinase Activity in Diaphragm Muscle in Response to Free Radical Scavenger Administration in Dystrophin-Deficient Mdx Mice
Am. J. Pathol., February 1, 2007; 170(2): 633 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. L. Weir, M. L. Oppizzi, M. D. Henry, A. Onishi, K. P. Campbell, M. J. Bissell, and J. L. Muschler
Dystroglycan loss disrupts polarity and {beta}-casein induction in mammary epithelial cells by perturbing laminin anchoring
J. Cell Sci., October 1, 2006; 119(19): 4047 - 4058.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Schneider, A. A. Khalil, J. Poulton, C. Castillejo-Lopez, D. Egger-Adam, A. Wodarz, W.-M. Deng, and S. Baumgartner
Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization
Development, October 1, 2006; 133(19): 3805 - 3815.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. S. Poulton and W.-M. Deng
Dystroglycan down-regulation links EGF receptor signaling and anterior-posterior polarity formation in the Drosophila oocyte
PNAS, August 22, 2006; 103(34): 12775 - 12780.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. P. Johnson, S. H. Kang, and J. M. Kramer
C. elegans dystroglycan DGN-1 functions in epithelia and neurons, but not muscle, and independently of dystrophin
Development, May 15, 2006; 133(10): 1911 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. R. Tremblay and S. Carbonetto
An Extracellular Pathway for Dystroglycan Function in Acetylcholine Receptor Aggregation and Laminin Deposition in Skeletal Myotubes
J. Biol. Chem., May 12, 2006; 281(19): 13365 - 13373.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Fukumoto, J. H. Miner, H. Ida, E. Fukumoto, K. Yuasa, H. Miyazaki, M. P. Hoffman, and Y. Yamada
Laminin {alpha}5 Is Required for Dental Epithelium Growth and Polarity and the Development of Tooth Bud and Shape
J. Biol. Chem., February 24, 2006; 281(8): 5008 - 5016.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Barresi and K. P. Campbell
Dystroglycan: from biosynthesis to pathogenesis of human disease
J. Cell Sci., January 15, 2006; 119(2): 199 - 207.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Lyalin, K. Koles, S. D. Roosendaal, E. Repnikova, L. Van Wechel, and V. M. Panin
The twisted Gene Encodes Drosophila Protein O-Mannosyltransferase 2 and Genetically Interacts With the rotated abdomen Gene Encoding Drosophila Protein O-Mannosyltransferase 1
Genetics, January 1, 2006; 172(1): 343 - 353.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhan, M. R. Tremblay, N. Melian, and S. Carbonetto
Evidence That Dystroglycan Is Associated with Dynamin and Regulates Endocytosis
J. Biol. Chem., May 6, 2005; 280(18): 18015 - 18024.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ichimiya, H. Manya, Y. Ohmae, H. Yoshida, K. Takahashi, R. Ueda, T. Endo, and S. Nishihara
The Twisted Abdomen Phenotype of Drosophila POMT1 and POMT2 Mutants Coincides with Their Heterophilic Protein O-Mannosyltransferase Activity
J. Biol. Chem., October 8, 2004; 279(41): 42638 - 42647.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Singh, Y. Itahana, S. Knight-Krajewski, M. Kanagawa, K. P. Campbell, M. J. Bissell, and J. Muschler
Proteolytic Enzymes and Altered Glycosylation Modulate Dystroglycan Function in Carcinoma Cells
Cancer Res., September 1, 2004; 64(17): 6152 - 6159.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Brumby, J. Secombe, J. Horsfield, M. Coombe, N. Amin, D. Coates, R. Saint, and H. Richardson
A Genetic Screen for Dominant Modifiers of a cyclin E Hypomorphic Mutation Identifies Novel Regulators of S-Phase Entry in Drosophila
Genetics, September 1, 2004; 168(1): 227 - 251.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Z. Morris, C. Navarro, and R. Lehmann
Identification and Analysis of Mutations in bob, Doa and Eight New Genes Required for Oocyte Specification and Development in Drosophila melanogaster
Genetics, August 1, 2003; 164(4): 1435 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. E. Michele and K. P. Campbell
Dystrophin-Glycoprotein Complex: Post-translational Processing and Dystroglycan Function
J. Biol. Chem., April 25, 2003; 278(18): 15457 - 15460.
[Full Text] [PDF]


© The Company of Biologists Ltd 2003