QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

doi: 10.1242/10.1242/dev.00488

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alvarez, A. D. Articles by Skeath, J. B. Search for Related Content PubMed PubMed Citation Articles by Alvarez, A. D. Articles by Skeath, J. B.

pannier and pointedP2 act sequentially to regulate Drosophila heart development

¹ Department of Genetics, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO 63110, USA
² Program in Developmental Biology, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO 63110, USA

^* Author for correspondence (email: jskeath{at}genetics.wustl.edu)

Accepted 17 March 2003

The Drosophila heart consists of two major cell types: cardioblasts, which form the contractile tube of the heart; and pericardial cells, which flank the cardioblasts and are thought to filter and detoxify the blood or hemolymph of the fly. We present the completion of the entire cell lineage of all heart cells. Notably, we detect a previously unappreciated distinction between the lineages of heart cells located in the posterior seven segments relative to those located more anteriorly. Using a genetic screen, we have identified the ETS-transcription factor pointed as a key regulator of cardioblast and pericardial cell fates in the posterior seven segments of the heart. In this domain, pointed promotes pericardial cell development and opposes cardioblast development. We find that this function of pointed is carried out primarily if not exclusively by the pointedP2 isoform and, that in this context, pointedP2 may act independently of Ras/MAPK pathway activity. We go on to show that the GATA transcription factor pannier acts early in dorsal mesoderm development to promote the development of the cardiac mesoderm and thus all heart cells. Finally, we demonstrate that pannier acts upstream of pointed in a developmental pathway in which pannier promotes cardiac mesoderm formation, and pointed acts subsequently in this domain to distinguish between cardioblast and pericardial cell fates.

Key words: Drosophila, Heart development, GATA factor, ETS-domain proteins

Related articles in Development:

Flying to the heart of heart development

Development 2003 130: 1304. [Full Text]  

This article has been cited by other articles:

				M. Zmojdzian, J. P. Da Ponte, and K. Jagla Cellular components and signals required for the cardiac outflow tract assembly in Drosophila PNAS, February 19, 2008; 105(7): 2475 - 2480. [Abstract] [Full Text] [PDF]

				S. G. Sprecher, F. Pichaud, and C. Desplan Adult and larval photoreceptors use different mechanisms to specify the same Rhodopsin fates Genes & Dev., September 1, 2007; 21(17): 2182 - 2195. [Abstract] [Full Text] [PDF]

				A. N. Johnson, L. A. Burnett, J. Sellin, A. Paululat, and S. J. Newfeld Defective Decapentaplegic Signaling Results in Heart Overgrowth and Reduced Cardiac Output in Drosophila Genetics, July 1, 2007; 176(3): 1609 - 1624. [Abstract] [Full Text] [PDF]

				M. J. Sukhanova, D. K. Deb, G. M. Gordon, M. T. Matakatsu, and W. Du Proneural Basic Helix-Loop-Helix Proteins and Epidermal Growth Factor Receptor Signaling Coordinately Regulate Cell Type Specification and cdk Inhibitor Expression during Development Mol. Cell. Biol., April 15, 2007; 27(8): 2987 - 2996. [Abstract] [Full Text] [PDF]

				J.-Y. Roignant, S. Hamel, F. Janody, and J. E. Treisman The novel SAM domain protein Aveugle is required for Raf activation in the Drosophila EGF receptor signaling pathway. Genes & Dev., April 1, 2006; 20(7): 795 - 806. [Abstract] [Full Text] [PDF]

				Z. Han, P. Yi, X. Li, and E. N. Olson Hand, an evolutionarily conserved bHLH transcription factor required for Drosophila cardiogenesis and hematopoiesis Development, March 15, 2006; 133(6): 1175 - 1182. [Abstract] [Full Text] [PDF]

				M. Fujioka, R. J. Wessells, Z. Han, J. Liu, K. Fitzgerald, G. L. Yusibova, M. Zamora, P. Ruiz-Lozano, R. Bodmer, and J. B. Jaynes Embryonic even skipped-Dependent Muscle and Heart Cell Fates Are Required for Normal Adult Activity, Heart Function, and Lifespan Circ. Res., November 25, 2005; 97(11): 1108 - 1114. [Abstract] [Full Text] [PDF]

				I. Reim and M. Frasch The Dorsocross T-box genes are key components of the regulatory network controlling early cardiogenesis in Drosophila Development, November 15, 2005; 132(22): 4911 - 4925. [Abstract] [Full Text] [PDF]

				Z. Han and E. N. Olson Hand is a direct target of Tinman and GATA factors during Drosophila cardiogenesis and hematopoiesis Development, August 1, 2005; 132(15): 3525 - 3536. [Abstract] [Full Text] [PDF]

				J. Wang, Y. Tao, I. Reim, K. Gajewski, M. Frasch, and R. A. Schulz Expression, Regulation, and Requirement of the Toll Transmembrane Protein during Dorsal Vessel Formation in Drosophila melanogaster Mol. Cell. Biol., May 15, 2005; 25(10): 4200 - 4210. [Abstract] [Full Text] [PDF]

				M. Zikova, J.-P. Da Ponte, B. Dastugue, and K. Jagla Patterning of the cardiac outflow region in Drosophila PNAS, October 14, 2003; 100(21): 12189 - 12194. [Abstract] [Full Text] [PDF]

				S. L. Klinedinst and R. Bodmer Gata factor Pannier is required to establish competence for heart progenitor formation Development, July 1, 2003; 130(13): 3027 - 3038. [Abstract] [Full Text] [PDF]