spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 24 September 2003
doi: 10.1242/dev.00737


This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Movies
Right arrow All Versions of this Article:
dev.00737v1
130/22/5425    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weaver, C.
Right arrow Articles by Kimelman, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weaver, C.
Right arrow Articles by Kimelman, D.
Development 130, 5425-5436 (2003)
Copyright © 2003 The Company of Biologists Limited

GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs

Carole Weaver1,2, Gist H. Farr, III1,*, Weijun Pan3,*, Brian A. Rowning4,*, Jiyong Wang3, Junhao Mao5, Dianqing Wu5, Lin Li3, Carolyn A. Larabell4 and David Kimelman1,{dagger}

1 Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
2 Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
3 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Shanghai, China
4 Department of Anatomy, University of California, San Francisco, CA 94143 and Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
5 Department of Genetics and Developmental Biology, University of Connecticut, Farmington, CN 06030, USA

{dagger} Author for correspondence (e-mail: kimelman{at}u.washington.edu)

Accepted 23 July 2003

In Xenopus, axis development is initiated by dorsally elevated levels of cytoplasmic ß-catenin, an intracellular factor regulated by GSK3 kinase activity. Upon fertilization, factors that increase ß-catenin stability are translocated to the prospective dorsal side of the embryo in a microtubule-dependent process. However, neither the identity of these factors nor the mechanism of their movement is understood. Here, we show that the GSK3 inhibitory protein GBP/Frat binds kinesin light chain (KLC), a component of the microtubule motor kinesin. Upon egg activation, GBP-GFP and KLC-GFP form particles and exhibit directed translocation. KLC, through a previously uncharacterized conserved domain, binds a region of GBP that is required for GBP translocation and for GSK3 binding, and competes with GSK3 for GBP. We propose a model in which conventional kinesin transports a GBP-containing complex to the future dorsal side, where GBP dissociates and contributes to the local stabilization of ß-catenin by binding and inhibiting GSK3.

Key words: Frat, Wnt pathway, Axis specification, Cortical rotation, Microtubules


Related articles in Development:

Motoring towards axis determination

Development 2003 130: 2204. [Full Text]  






© The Company of Biologists Ltd 2003