spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 2 June 2004
doi: 10.1242/dev.01173

Development 131, 3133-3145 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
dev.01173v1
131/13/3133    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyata, T.
Right arrow Articles by Ogawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyata, T.
Right arrow Articles by Ogawa, M.

Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells

Takaki Miyata1,2,3,{dagger}, Ayano Kawaguchi1,*, Kanako Saito1,2,3, Masako Kawano1, Tetsuji Muto1 and Masaharu Ogawa1,3

1 Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, Saitama 351-0198, Japan
2 Department of Anatomy and Cell Biology, Graduate School of Medicine, Nagoya University, Nagoya 466-8550, Japan
3 CREST, Japan Science and Technology Corporation (JST), Tokyo 103-0027, Japan

{dagger} Author for correspondence (e-mail: tmiyata{at}med.nagoya-u.ac.jp)

Accepted 16 March 2004

Mature neocortical layers all derive from the cortical plate (CP), a transient zone in the dorsal telencephalon into which young neurons are continuously delivered. To understand cytogenetic and histogenetic events that trigger the emergence of the CP, we have used a slice culture technique. Most divisions at the ventricular surface generated paired cycling daughters (P/P divisions) and the majority of the P/P divisions were asymmetric in daughter cell behavior; they frequently sent one daughter cell to a non-surface (NS) position, the subventricular zone (SVZ), within a single cell-cycle length while keeping the other mitotic daughter for division at the surface. The NS-dividing cells were mostly Hu+ and their daughters were also Hu+, suggesting their commitment to the neuronal lineage and supply of early neurons at a position much closer to their destiny than from the ventricular surface. The release of a cycling daughter cell to SVZ was achieved by collapse of the ventricular process of the cell, followed by its NS division. Neurogenin2 (Ngn2) was immunohistochemically detected in a certain cycling population during G1 phase and was further restricted during G2-M phases to the SVZ-directed population. Its retroviral introduction converted surface divisions to NS divisions. The asymmetric P/P division may therefore contribute to efficient neuron/progenitor segregation required for CP initiation through cell cycle-dependent and lineage-restricted expression of Ngn2.

Key words: Cerebral cortex, Cortical plate, Neuroepithelium, Subventricular zone, Asymmetric cell division, Cell migration, Cell cycle, Cell fate determination, Layer formation, Neurogenin2, Slice culture, Mouse






© The Company of Biologists Ltd 2004