|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 17 December 2003
doi: 10.1242/dev.00918
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Laboratoire de Méiose et de Maturation Gamétique, DRR / DSV/ CEA
– U566 INSERM – Université Paris 7, Fontenay aux Roses,
France
2 Laboratoire de Radiosensibilité des Cellules Germinales, DRR / DSV/ CEA
– U566 INSERM – Université Paris 7, Fontenay aux Roses,
France
3 CNRS FRE 2358, Génétique Expérimentale et
Moléculaire, Orléans, France
* Author for correspondence (e-mail: pfouchet{at}armoise.saclay.cea.fr)
Accepted 13 October 2003
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be
identified by the `Side Population' marker based on Hoechst 33342 efflux. We
show that mouse testicular cells also display a `Side Population' that express
Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in
hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor
Ko143 show that germinal `Side Population' phenotype is dependent on BCRP1
activity. Analysis of two well-defined models of altered spermatogenesis
(W/Wv mutants and cryptorchid male mice) and RNA
expression studies of differentiation markers demonstrate that germinal `Side
Population' contains spermatogonial cells. In addition,
6-integrin and Stra8 germinal stem cell markers, are
expressed in the `Side Population'. In vivo repopulation assay clearly
establishes that testis `Side Population' in adult mice is highly enriched in
male germ stem cells.
Key words: Spermatogenesis, Stem cells, BCRP1/ABCG2, Transplantation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  B. P. Hermann, M. Sukhwani, D. R. Simorangkir, T. Chu, T. M. Plant, and K. E. Orwig Molecular dissection of the male germ cell lineage identifies putative spermatogonial stem cells in rhesus macaques Hum. Reprod., July 1, 2009; 24(7): 1704 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Pfister, A. Oikonomopoulos, K.-I. Sereti, R. L. Sohn, D. Cullen, G. C. Fine, F. Mouquet, K. Westerman, and R. Liao Role of the ATP-Binding Cassette Transporter Abcg2 in the Phenotype and Function of Cardiac Side Population Cells Circ. Res., October 10, 2008; 103(8): 825 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kobayashi, K. Saito, T. Moritomo, K. Araki, F. Takizawa, and T. Nakanishi Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue Blood, February 1, 2008; 111(3): 1131 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rubinek, V. Chesnokova, I. Wolf, K. Wawrowsky, G. Vlotides, and S. Melmed Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1082 - C1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bhattacharya, A. Das, K. Mallya, and I. Ahmad Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling J. Cell Sci., August 1, 2007; 120(15): 2652 - 2662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. X. Liang, R. Summer, X. Sun, and A. Fine Gene expression profiling and localization of Hoechst-effluxing CD45- and CD45+ cells in the embryonic mouse lung Physiol Genomics, October 17, 2005; 23(2): 172 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P.A. van Bragt, N. Ciliberti, W. L. Stanford, D. G. de Rooij, and A. M.M. van Pelt LY6A/E (SCA-1) Expression in the Mouse Testis Biol Reprod, October 1, 2005; 73(4): 634 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Riou, H. Bastos, B. Lassalle, M. Coureuil, J. Testart, F. D. Boussin, I. Allemand, and P. Fouchet The Telomerase Activity of Adult Mouse Testis Resides in the Spermatogonial {alpha}6-Integrin-Positive Side Population Enriched in Germinal Stem Cells Endocrinology, September 1, 2005; 146(9): 3926 - 3932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Patrawala, T. Calhoun, R. Schneider-Broussard, J. Zhou, K. Claypool, and D. G. Tang Side Population Is Enriched in Tumorigenic, Stem-Like Cancer Cells, whereas ABCG2+ and ABCG2- Cancer Cells Are Similarly Tumorigenic Cancer Res., July 15, 2005; 65(14): 6207 - 6219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Falender, R. N. Freiman, K. G. Geles, K. C. Lo, K. Hwang, D. J. Lamb, P. L. Morris, R. Tjian, and J. S. Richards Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID Genes & Dev., April 1, 2005; 19(7): 794 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Lo, V. M. Brugh III, M. Parker, and D. J. Lamb Isolation and Enrichment of Murine Spermatogonial Stem Cells Using Rhodamine 123 Mitochondrial Dye Biol Reprod, March 1, 2005; 72(3): 767 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. de Rooij and M. P. A. van Bragt Leydig Cells: Testicular Side Population Harbors Transplantable Leydig Stem Cells Endocrinology, September 1, 2004; 145(9): 4009 - 4010. [Full Text] [PDF]
|
|
|
|
|
|
K. C. Lo, Z. Lei, Ch. V. Rao, J. Beck, and D. J. Lamb De Novo Testosterone Production in Luteinizing Hormone Receptor Knockout Mice after Transplantation of Leydig Stem Cells Endocrinology, September 1, 2004; 145(9): 4011 - 4015. [Abstract] [Full Text] [PDF]
|
|
