|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 15 September 2004
doi: 10.1242/dev.01389
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Division of Mammalian Development, National Institute for Medical Research,
The Ridgeway, Mill Hill, London NW7 1AA, UK
2 Division of Developmental Biology, National Institute for Medical Research,
The Ridgeway, Mill Hill, London NW7 1AA, UK
** Author for correspondence (e-mail: a.rana{at}gurdon.cam.ac.uk)
Accepted 23 July 2004
Dyneins have been implicated in left-right axis determination during embryonic development and in a variety of human genetic syndromes. In this paper, we study the recently discovered mouse dynein 2 light intermediate chain (mD2LIC), which is believed to be involved in retrograde intraflagella transport and which, like left-right dynein, is expressed in the node of the mouse embryo. Cells of the ventral node of mouse embryos lacking mD2LIC have an altered morphology and lack monocilia, and expression of Foxa2 and Shh in this structure is reduced or completely absent. At later stages, consistent with the absence of nodal cilia, mD2LIC is required for the establishment of the left-right axis and for normal expression of Nodal, and the ventral neural tube fails to express Shh, Foxa2 and Ebaf. mD2LIC also functions indirectly in the survival of anterior definitive endoderm and in the maintenance of the anterior neural ridge, probably through maintenance of Foxa2/Hnf3ß expression. Together, our results indicate that mD2LIC is required to maintain or establish ventral cell fates and for correct signalling by the organiser and midline, and they identify the first embryonic function of a vertebrate cytoplasmic dynein.
Key words: D2LIC, Axis formation, Handedness, Mouse embryo, Intra-flagellar transport, Lefty2, Hnf3ß
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  B. L. Krock, I. Mills-Henry, and B. D. Perkins Retrograde Intraflagellar Transport by Cytoplasmic Dynein-2 Is Required for Outer Segment Extension in Vertebrate Photoreceptors but Not Arrestin Translocation Invest. Ophthalmol. Vis. Sci., November 1, 2009; 50(11): 5463 - 5471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Huang and A. F. Schier Dampened Hedgehog signaling but normal Wnt signaling in zebrafish without cilia Development, September 15, 2009; 136(18): 3089 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Rajagopalan, A. Subramanian, D. E. Wilkes, D. G. Pennock, and D. J. Asai Dynein-2 Affects the Regulation of Ciliary Length but Is Not Required for Ciliogenesis in Tetrahymena thermophila Mol. Biol. Cell, January 1, 2009; 20(2): 708 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mische, Y. He, L. Ma, M. Li, M. Serr, and T. S. Hays Dynein Light Intermediate Chain: An Essential Subunit That Contributes to Spindle Checkpoint Inactivation Mol. Biol. Cell, November 1, 2008; 19(11): 4918 - 4929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ait-Lounis, D. Baas, E. Barras, C. Benadiba, A. Charollais, R. Nlend Nlend, D. Liegeois, P. Meda, B. Durand, and W. Reith Novel Function of the Ciliogenic Transcription Factor RFX3 in Development of the Endocrine Pancreas Diabetes, April 1, 2007; 56(4): 950 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Bisgrove and H. J. Yost The roles of cilia in developmental disorders and disease Development, November 1, 2006; 133(21): 4131 - 4143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Michaud and B. K. Yoder The Primary Cilium in Cell Signaling and Cancer. Cancer Res., July 1, 2006; 66(13): 6463 - 6467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Huangfu and K. V. Anderson From the Cover: Cilia and Hedgehog responsiveness in the mouse PNAS, August 9, 2005; 102(32): 11325 - 11330. [Abstract] [Full Text] [PDF]
|
|
