|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 15 September 2004
doi: 10.1242/dev.01349
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Developmental Genetics Program and the Department of Cell Biology, The
Skirball Institute of Biomolecular Medicine, New York University Medical
Center, 540 First Avenue, New York, NY 10016, USA
2 Harvard University, Department of Molecular and Cellular Biology, Cambridge,
MA 02138, USA
* Author for correspondence (e-mail: fishell{at}saturn.med.nyu.edu)
Accepted 13 July 2004
Hedgehog signaling is required for multiple aspects of brain development, including growth, the establishment of both dorsal and ventral midline patterning and the generation of specific cell types such as oligodendrocytes and interneurons. To identify more precisely when during development hedgehog signaling mediates these events, we directed the removal of hedgehog signaling within the brain by embryonic day 9 of development, using a FoxG1Cre driver line to mediate the removal of a conditional smoothened null allele. We observed a loss of ventral telencephalic patterning that appears to result from an initial lack of specification of these structures rather than by changes in proliferation or cell death. A further consequence of the removal of smoothened in these mice is the near absence of both oligodendrocytes and interneurons. Surprisingly, the dorsal midline appears to be patterned normally in these mutants. Together with previous analyses, the present results demonstrate that hedgehog signaling in the period between E9.0 and E12 is essential for the patterning of ventral regions and the generation of cell types that are thought to largely arise from them.
Key words: Telencephalon, Sonic hedgehog, Patterning, Oligodendrocyte, Interneuron
This article has been cited by other articles:
|
|
 |
|
  N. Warr, N. Powles-Glover, A. Chappell, J. Robson, D. Norris, and R. M. Arkell Zic2-associated holoprosencephaly is caused by a transient defect in the organizer region during gastrulation Hum. Mol. Genet., October 1, 2008; 17(19): 2986 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Tucker, S. Segall, D. Gopalakrishna, Y. Wu, M. Vernon, F. Polleux, and A.-S. LaMantia Molecular Specification and Patterning of Progenitor Cells in the Lateral and Medial Ganglionic Eminences J. Neurosci., September 17, 2008; 28(38): 9504 - 9518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Komada, H. Saitsu, M. Kinboshi, T. Miura, K. Shiota, and M. Ishibashi Hedgehog signaling is involved in development of the neocortex Development, August 15, 2008; 135(16): 2717 - 2727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Arbour, J. L. Vanderluit, J. N. Le Grand, A. Jahani-Asl, V. A. Ruzhynsky, E. C. C. Cheung, M. A. Kelly, A. E. MacKenzie, D. S. Park, J. T. Opferman, et al. Mcl-1 Is a Key Regulator of Apoptosis during CNS Development and after DNA Damage J. Neurosci., June 11, 2008; 28(24): 6068 - 6078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Balordi and G. Fishell Mosaic Removal of Hedgehog Signaling in the Adult SVZ Reveals That the Residual Wild-Type Stem Cells Have a Limited Capacity for Self-Renewal J. Neurosci., December 26, 2007; 27(52): 14248 - 14259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. B. Butt, I. Cobos, J. Golden, N. Kessaris, V. Pachnis, and S. Anderson Transcriptional Regulation of Cortical Interneuron Development J. Neurosci., October 31, 2007; 27(44): 11847 - 11850. [Full Text] [PDF]
|
|
|
|
|
|
B. G. Rash and E. A. Grove Patterning the Dorsal Telencephalon: A Role for Sonic Hedgehog? J. Neurosci., October 24, 2007; 27(43): 11595 - 11603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Huang, Y. Litingtung, and C. Chiang Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord Development, June 1, 2007; 134(11): 2095 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Ruzhynsky, K. A. McClellan, J. L. Vanderluit, Y. Jeong, M. Furimsky, D. S. Park, D. J. Epstein, V. A. Wallace, and R. S. Slack Cell Cycle Regulator E2F4 Is Essential for the Development of the Ventral Telencephalon J. Neurosci., May 30, 2007; 27(22): 5926 - 5935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Balordi and G. Fishell Hedgehog Signaling in the Subventricular Zone Is Required for Both the Maintenance of Stem Cells and the Migration of Newborn Neurons J. Neurosci., May 30, 2007; 27(22): 5936 - 5947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gutin, M. Fernandes, L. Palazzolo, H. Paek, K. Yu, D. M. Ornitz, S. K. McConnell, and J. M. Hebert FGF signalling generates ventral telencephalic cells independently of SHH Development, August 1, 2006; 133(15): 2937 - 2946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Blaess, J. D. Corrales, and A. L. Joyner Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region Development, May 1, 2006; 133(9): 1799 - 1809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jeong, K. El-Jaick, E. Roessler, M. Muenke, and D. J. Epstein A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers Development, February 15, 2006; 133(4): 761 - 772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Xu, C. P. Wonders, and S. A. Anderson Sonic hedgehog maintains the identity of cortical interneuron progenitors in the ventral telencephalon Development, November 15, 2005; 132(22): 4987 - 4998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Mason, S. M. Rakowiecki, M. Raftopoulou, S. Nery, Y. Huang, T. Gridley, and G. Fishell Notch signaling coordinates the patterning of striatal compartments Development, October 1, 2005; 132(19): 4247 - 4258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Spoelgen, A. Hammes, U. Anzenberger, D. Zechner, O. M. Andersen, B. Jerchow, and T. E. Willnow LRP2/megalin is required for patterning of the ventral telencephalon Development, January 15, 2005; 132(2): 405 - 414. [Abstract] [Full Text] [PDF]
|
|
