|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 29 September 2004
doi: 10.1242/dev.01314
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305-5329, USA
Author for correspondence (e-mail:
fuller{at}cmgm.stanford.edu)
Accepted 17 June 2004
Alternate forms of the PolII transcription initiation machinery have been proposed to play a role in selective activation of cell-type-specific gene expression programs during cellular differentiation. The cannonball (can) gene of Drosophila encodes a homolog of a TBP-associated factor (dTAF5) protein expressed only in spermatocytes, where it is required for normal transcription of genes required for spermatid differentiation. We show that Drosophila primary spermatocytes also express four additional tissue-specific TAFs: nht (homolog of dTAF4), mia (homolog of dTAF6), sa (homolog of dTAF8) and rye (homolog of dTAF12). Mutations in nht, mia and sa have similar effects in primary spermatocytes on transcription of several target genes involved in spermatid differentiation, and cause the same phenotypes as mutations in can, blocking both meiotic cell cycle progression and spermatid differentiation. The nht, mia, sa and rye proteins contain histone fold domain dimerization motifs. The nht and rye proteins interact structurally when co-expressed in bacteria, similarly to their generally expressed homologs TAF4 and TAF12, which heterodimerize. Strikingly, the structural interaction is tissue specific: nht did not interact with dTAF12 and dTAF4 did not interact with rye in a bacterial co-expression assay. We propose that the products of the five Drosophila genes encoding testis TAF homologs collaborate in an alternative TAF-containing protein complex to regulate a testis-specific gene expression program in primary spermatocytes required for terminal differentiation of male germ cells.
Key words: TAF, TFIID, Drosophila, Spermatogenesis, Spermatocyte, Transcription
This article has been cited by other articles:
|
|
 |
|
  M.D.E. Deato and R. Tjian An Unexpected Role of TAFs and TRFs in Skeletal Muscle Differentiation: Switching Core Promoter Complexes Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.028v1. [Abstract] [PDF]
|
|
|
|
 |
|
 L. M. Mikhaylova, K. Nguyen, and D. I. Nurminsky Analysis of the Drosophila melanogaster Testes Transcriptome Reveals Coordinate Regulation of Paralogous Genes Genetics, May 1, 2008; 179(1): 305 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhong and J. M. Belote The testis-specific proteasome subunit Pros{alpha}6T of D. melanogaster is required for individualization and nuclear maturation during spermatogenesis Development, October 1, 2007; 134(19): 3517 - 3525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. E. Deato and R. Tjian Switching of the core transcription machinery during myogenesis Genes & Dev., September 1, 2007; 21(17): 2137 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman, and S. DiNardo A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes Development, August 1, 2007; 134(15): 2851 - 2861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Baker and M. T. Fuller Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog Development, August 1, 2007; 134(15): 2863 - 2869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Robles, J. S. Wampole, M. J. Christians, and P. B. Larsen Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction J. Exp. Bot., July 1, 2007; 58(10): 2627 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Muller, M. A. Demeny, and L. Tora New Problems in RNA Polymerase II Transcription Initiation: Matching the Diversity of Core Promoters with a Variety of Promoter Recognition Factors J. Biol. Chem., May 18, 2007; 282(20): 14685 - 14689. [Full Text] [PDF]
|
|
|
|
|
|
E. L. Beall, P. W. Lewis, M. Bell, M. Rocha, D. L. Jones, and M. R. Botchan Discovery of tMAC: a Drosophila testis-specific meiotic arrest complex paralogous to Myb-Muv B Genes & Dev., April 15, 2007; 21(8): 904 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jiang, E. Benson, N. Bausek, K. Doggett, and H. White-Cooper Tombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with Always early Development, April 15, 2007; 134(8): 1549 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Cheng, M. G. Buffone, M. Kouadio, M. Goodheart, D. C. Page, G. L. Gerton, I. Davidson, and P. J. Wang Abnormal Sperm in Mice Lacking the Taf7l Gene Mol. Cell. Biol., April 1, 2007; 27(7): 2582 - 2589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Garbett, M. K. Tripathi, B. Cencki, J. H. Layer, and P. A. Weil Yeast TFIID Serves as a Coactivator for Rap1p by Direct Protein-Protein Interaction Mol. Cell. Biol., January 1, 2007; 27(1): 297 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Katzenberger, M. S. Marengo, and D. A. Wassarman ATM and ATR Pathways Signal Alternative Splicing of Drosophila TAF1 Pre-mRNA in Response to DNA Damage Mol. Cell. Biol., December 15, 2006; 26(24): 9256 - 9267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Metcalf and D. A. Wassarman DNA Binding Properties of TAF1 Isoforms with Two AT-hooks J. Biol. Chem., October 6, 2006; 281(40): 30015 - 30023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Kopytova, A. N. Krasnov, M. R. Kopantceva, E. N. Nabirochkina, J. V. Nikolenko, O. Maksimenko, M. M. Kurshakova, L. A. Lebedeva, M. M. Yerokhin, O. B. Simonova, et al. Two Isoforms of Drosophila TRF2 Are Involved in Embryonic Development, Premeiotic Chromatin Condensation, and Proper Differentiation of Germ Cells of Both Sexes. Mol. Cell. Biol., October 1, 2006; 26(20): 7492 - 7505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Arya, M. J. P. Lodico, O. I. Ahmad, R. Amin, and J. E. Tomkiel Molecular Characterization of teflon, a Gene Required for Meiotic Autosome Segregation in Male Drosophila melanogaster Genetics, September 1, 2006; 174(1): 125 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Wright, M. T. Marr II, and R. Tjian TAF4 nucleates a core subcomplex of TFIID and mediates activated transcription from a TATA-less promoter PNAS, August 15, 2006; 103(33): 12347 - 12352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Mikhaylova, A. M. Boutanaev, and D. I. Nurminsky Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line PNAS, August 8, 2006; 103(32): 11975 - 11980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Marr II, Y. Isogai, K. J. Wright, and R. Tjian Coactivator cross-talk specifies transcriptional output Genes & Dev., June 1, 2006; 20(11): 1458 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Stouffs, A. Willems, W. Lissens, H. Tournaye, A. Van Steirteghem, and I. Liebaers The role of the testis-specific gene hTAF7L in the aetiology of male infertility Mol. Hum. Reprod., April 1, 2006; 12(4): 263 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Geles, R. N. Freiman, W.-L. Liu, S. Zheng, E. Voronina, and R. Tjian Cell-type-selective induction of c-jun by TAF4b directs ovarian-specific transcription networks PNAS, February 21, 2006; 103(8): 2594 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Krasnov, M. M. Kurshakova, V. E. Ramensky, P. V. Mardanov, E. N. Nabirochkina, and S. G. Georgieva A retrocopy of a gene can functionally displace the source gene in evolution Nucleic Acids Res., November 27, 2005; 33(20): 6654 - 6661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, M. Hiller, Y. Sancak, and M. T. Fuller Tissue-Specific TAFs Counteract Polycomb to Turn on Terminal Differentiation Science, November 4, 2005; 310(5749): 869 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Frontini, E. Soutoglou, M. Argentini, C. Bole-Feysot, B. Jost, E. Scheer, and L. Tora TAF9b (Formerly TAF9L) Is a Bona Fide TAF That Has Unique and Overlapping Roles with TAF9 Mol. Cell. Biol., June 1, 2005; 25(11): 4638 - 4649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Falender, R. N. Freiman, K. G. Geles, K. C. Lo, K. Hwang, D. J. Lamb, P. L. Morris, R. Tjian, and J. S. Richards Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID Genes & Dev., April 1, 2005; 19(7): 794 - 803. [Abstract] [Full Text] [PDF]
|
|
