QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 3 March 2004
doi: 10.1242/dev.01047

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.01047v1 131/7/1629    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, S. Articles by Sze, J. Y. Search for Related Content PubMed PubMed Citation Articles by Zhang, S. Articles by Sze, J. Y. Social Bookmarking                         What's this?

Caenorhabditis elegans TRPV ion channel regulates 5HT biosynthesis in chemosensory neurons

Department of Anatomy and Neurobiology, College of Medicine, University of California Irvine, Irvine, CA 92697-4040, USA

^* Author for correspondence (e-mail: jsze{at}uci.edu)

Accepted 19 December 2003

Serotonin (5HT) is a pivotal signaling molecule that modulates behavioral and endocrine responses to diverse chemical and physical stimuli. We report cell-specific regulation of 5HT biosynthesis by transient receptor potential V (TRPV) ion channels in C. elegans. Mutations in the TRPV genes osm-9 or ocr-2 dramatically downregulate the expression of the gene encoding the 5HT synthesis enzyme tryptophan hydroxylase (tph-1) in the serotonergic chemosensory neurons ADF, but neither the mutation nor the double mutation of both channel genes affects other types of serotonergic neurons. The TRPV genes are expressed in the ADF neurons but not in other serotonergic neurons, and act cell-autonomously to regulate a neuron-specific transcription program. Whereas in olfactory neurons OSM-9 and OCR-2 function is dependent on ODR-3 G, the activity of ODR-3 or two other G proteins expressed in the ADF neurons is not required for upregulating tph-1 expression, thus the TRPV ion channels in different neurons may be regulated by different mechanisms. A gain-of-function mutation in CaMKII UNC-43 partially suppresses the downregulation of tph-1 in the TRPV mutants, thus CaMKII may be an effector of the TRPV signaling. Mutations in the TRPV genes cause worms developmentally arrest at the Dauer stage. This developmental defect is due in part to reduced 5HT inputs into daf-2/insulin neuroendocrine signaling.

Key words: C. elegans, Transcription, Serotonin biosynthesis, TRPV ion channels, Metabolic control

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Moussaif and J. Y. Sze Intraflagellar Transport/Hedgehog-Related Signaling Components Couple Sensory Cilium Morphology and Serotonin Biosynthesis in Caenorhabditis elegans J. Neurosci., April 1, 2009; 29(13): 4065 - 4075. [Abstract] [Full Text] [PDF]

				N. Fielenbach and A. Antebi C. elegans dauer formation and the molecular basis of plasticity Genes & Dev., August 15, 2008; 22(16): 2149 - 2165. [Abstract] [Full Text] [PDF]

				J. E. Tanis, J. J. Moresco, R. A. Lindquist, and M. R. Koelle Regulation of Serotonin Biosynthesis by the G Proteins G{alpha}o and G{alpha}q Controls Serotonin Signaling in Caenorhabditis elegans Genetics, January 1, 2008; 178(1): 157 - 169. [Abstract] [Full Text] [PDF]

				W. Liedtke Transient receptor potential vanilloid channels functioning in transduction of osmotic stimuli J. Endocrinol., December 1, 2006; 191(3): 515 - 523. [Abstract] [Full Text] [PDF]

				E. A. Bates, M. Victor, A. K. Jones, Y. Shi, and A. C. Hart Differential contributions of Caenorhabditis elegans histone deacetylases to huntingtin polyglutamine toxicity. J. Neurosci., March 8, 2006; 26(10): 2830 - 2838. [Abstract] [Full Text] [PDF]

				W. Liedtke TRPV4 plays an evolutionary conserved role in the transduction of osmotic and mechanical stimuli in live animals J. Physiol., August 15, 2005; 567(1): 53 - 58. [Abstract] [Full Text] [PDF]