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First published online 27 April 2005
doi: 10.1242/dev.01846

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Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages

¹ Department of Cell and Matrix Biology, MCRI, 3052 Parkville Victoria, Australia
² Department of Experimental Medicine I, University Erlangen-Nürnberg, 91054 Erlangen, Germany
³ Department of Ophthalmology, University Erlangen-Nürnberg, 91054 Erlangen, Germany
⁴ Max-Planck-Institute of Psychiatry, 80804 München, Germany
⁵ Department of Experimental Pathology, Lund University, 22363 Lund, Sweden
⁶ Department of Genetics, University Erlangen-Nürnberg, 91054 Erlangen, Germany

^* Author for correspondence (e-mail: bent.brachvogel{at}mcri.edu.au)

Accepted 22 March 2005

The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system. In this paper, the expression of an Anxa5-lacZ fusion gene was used to define the onset of expression in the vasculature and to characterize these Anxa5-lacZ-expressing vasculature-associated cells. After blastocyst implantation, Anxa5-lacZ-positive cells were first detected in extra-embryonic tissues and in angioblast progenitors forming the primary vascular plexus. Later, expression is highly restricted to perivascular cells in most blood vessels resembling pericytes or vascular smooth muscle cells. Viable Anxa5-lacZ⁺ perivascular cells were isolated from embryos as well as adult brain meninges by specific staining with fluorescent X-gal substrates and cell-sorting. These purified lacZ⁺ cells specifically express known markers of pericytes, but also markers characteristic for stem cell populations. In vitro and in vivo differentiation experiments show that this cell pool expresses early markers of chondrogenesis, is capable of forming a calcified matrix and differentiates into adipocytes. Hence, Anxa5 expression in perivascular cells from mouse defines a novel population of cells with a distinct developmental potential.

Key words: Annexin A5, Perivascular cells, Pericytes, Adult stem cells

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