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First published online 15 June 2005
doi: 10.1242/dev.01900

Development 132, 3185-3195 (2005)
Published by The Company of Biologists 2005
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Essential kinase-independent role of a Fer-like non-receptor tyrosine kinase in Caenorhabditis elegans morphogenesis

Aaron P. Putzke, Sherry T. Hikita, Dennis O. Clegg and Joel H. Rothman*

Neuroscience Research Institute and Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA

* Author for correspondence (e-mail: rothman{at}lifesci.ucsb.edu)

Accepted 12 May 2005

Morphogenesis requires coordination of cell surface activity and cytoskeletal architecture. During the initial stage of morphogenesis in Caenorhabditis elegans, the concerted movement of surface epithelial cells results in enclosure of the embryo by the epidermis. We report that Fer-related kinase-1 (FRK-1), an ortholog of the mammalian non-receptor tyrosine kinase Fer, is necessary for embryonic enclosure and morphogenesis in C. elegans. Expression of FRK-1 in epidermal cells is sufficient to rescue a chromosomal deficiency that removes the frk-1 locus, demonstrating its autonomous requirement in the epidermis. The essential function of FRK-1 is independent of its kinase domain, suggesting a non-enzymatic role in morphogenesis. Localization of FRK-1 to the plasma membrane requires ß-catenin, but not cadherin or {alpha}-catenin, and muscle-expressed ß-integrin is non-autonomously required for this localization; in the absence of these components FRK-1 becomes nuclear. Mouse FerT rescues the morphogenetic defects of frk-1 mutants and expression of FRK-1 in mammalian cells results in loss of adhesion, implying a conserved function for FRK-1/FerT in cell adhesion and morphogenesis. Thus, FRK-1 performs a kinase-independent function in differentiation and morphogenesis of the C. elegans epidermis during embryogenesis.

Key words: C. elegans, Fer kinase, Morphogenesis, Adhesion, ß-catenin


Related articles in Development:

Enclosed: the conserved non-kinase roles of FRK-1 kinase

Development 2005 132: e1401. [Full Text]  





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