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First published online 20 July 2005
doi: 10.1242/dev.01933

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.01933v1 132/16/3587    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fong, S. H. Articles by Korzh, V. Search for Related Content PubMed PubMed Citation Articles by Fong, S. H. Articles by Korzh, V.

Wnt signalling mediated by Tbx2b regulates cell migration during formation of the neural plate

Steven H. Fong^1,*, Alexander Emelyanov^1,*, Cathleen Teh¹ and Vladimir Korzh^1,2,

¹ Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, 138673 Singapore
² Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, 119260 Singapore

Author for correspondence (e-mail: vlad{at}imcb.a-star.edu.sg)

Accepted 9 June 2005

During gastrulation, optimal adhesion and receptivity to signalling cues are essential for cells to acquire new positions and identities via coordinated cell movements. T-box transcription factors and the Wnt signalling pathways are known to play important roles in these processes. Zebrafish tbx2b, a member of the TBX2 family, has previously been shown to be required for the specification of midline mesoderm. We show here that tbx2b transcripts are present during mid-gastrula before its expression is detected by whole-mount in situ hybridization. Isolated ectodermal cells deficient in Tbx2b have altered cell surface properties and the level of cadherins in these cells is lower. In chimaeric embryos generated by cell transplantation and single blastomere injections, Tbx2b-deficient cells are defective in cell movement in a cell-autonomous manner, resulting in their exclusion from the developing neural plate. Using this `exclusion' phenotype as a screen, we show that Tbx2b acts within the context of Fz7 signalling. The exclusion of cells lacking T-box proteins in chimeras during development was demonstrated with other T-box genes and may indicate a general functional mechanism for T-box proteins.

Key words: T-box, Cell adhesion, Zebrafish, Frizzled 7, Dishevelled, DIX domain, ß-catenin

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