QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 16 March 2005
doi: 10.1242/dev.01729

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.01729v1 132/8/1773    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maekawa, M. Articles by Nishida, E. Search for Related Content PubMed PubMed Citation Articles by Maekawa, M. Articles by Nishida, E. Social Bookmarking                         What's this?

Requirement of the MAP kinase signaling pathways for mouse preimplantation development

Momoko Maekawa¹, Takuya Yamamoto¹, Takuji Tanoue^1,*, Yasuhito Yuasa², Osamu Chisaka¹ and Eisuke Nishida^1,

¹ Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
² Department of Molecular Oncology, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan

Author for correspondence (e-mail: L50174{at}sakura.kudpc.kyoto-u.ac.jp)

Accepted 31 January 2005

Mammalian preimplantation development involves several crucial events, such as compaction and blastocyst formation, but little is known about essential genes that regulate this developmental process. Here, we have focused on MAP kinase signaling pathways as potential regulatory pathways for the process. Our results show that inhibition of the JNK pathway or of the p38 MAP kinase pathway, but not of the ERK pathway, results in inhibition of cavity formation, and that JNK and p38 are active during mouse preimplantation development. Our subsequent microarray analyses show that, of about 39,000 transcripts analyzed, the number of those genes whose expression level is sensitive to the inhibition of the JNK or the p38 pathway, but insensitive to the inhibition of the ERK pathway, is only 156. Moreover, of the 156 genes, expression of 10 genes (two genes upregulated and eight genes downregulated) is sensitive to either inhibition of the JNK or p38 pathways. These 10 genes include several genes known for their function in axis and pattern formation. Downregulation of some of the 10 genes simultaneously using siRNA leads to abnormality in cavity formation. Thus, this study has successfully narrowed down candidate genes of interest, detailed analysis of which will probably lead to elucidation of the molecular mechanism of preimplantation development.

Key words: JNK, Microarray analysis, p38, Preimplantaion development, Signal transduction

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Zuccotti, V. Merico, L. Sacchi, M. Bellone, T. C. Brink, M. Stefanelli, C. A. Redi, R. Bellazzi, J. Adjaye, and S. Garagna Oct-4 regulates the expression of Stella and Foxj2 at the Nanog locus: implications for the developmental competence of mouse oocytes Hum. Reprod., September 1, 2009; 24(9): 2225 - 2237. [Abstract] [Full Text] [PDF]

				N. L. Manuylov, F. O. Smagulova, L. Leach, and S. G. Tevosian Ovarian development in mice requires the GATA4-FOG2 transcription complex Development, November 15, 2008; 135(22): 3731 - 3743. [Abstract] [Full Text] [PDF]

				Wenjing Zhong, Yufen Xie, Yingchun Wang, J. Lewis, A. Trostinskaia, Fangfei Wang, E. E. Puscheck, and D. A. Rappolee Use of Hyperosmolar Stress to Measure Stress-Activated Protein Kinase Activation and Function in Human HTR Cells and Mouse Trophoblast Stem Cells Reproductive Sciences, September 1, 2007; 14(6): 534 - 547. [Abstract] [PDF]

				M. Maekawa, T. Yamamoto, M. Kohno, M. Takeichi, and E. Nishida Requirement for ERK MAP kinase in mouse preimplantation development Development, August 1, 2007; 134(15): 2751 - 2759. [Abstract] [Full Text] [PDF]

				S. Sudheer and J. Adjaye Functional genomics of human pre-implantation development Brief Funct Genomic Proteomic, July 31, 2007; (2007) elm012v1. [Abstract] [Full Text] [PDF]

				Y. Xie, W. Zhong, Y. Wang, A. Trostinskaia, F. Wang, E.E. Puscheck, and D.A. Rappolee Using hyperosmolar stress to measure biologic and stress-activated protein kinase responses in preimplantation embryos Mol. Hum. Reprod., July 1, 2007; 13(7): 473 - 481. [Abstract] [Full Text] [PDF]

				Y. Xie, F. Wang, W. Zhong, E. Puscheck, H. Shen, and D.A. Rappolee Shear Stress Induces Preimplantation Embryo Death That Is Delayed by the Zona Pellucida and Associated with Stress-Activated Protein Kinase-Mediated Apoptosis Biol Reprod, July 1, 2006; 75(1): 45 - 55. [Abstract] [Full Text] [PDF]

				K. Nagino, O. Nomura, Y. Takii, A. Myomoto, M. Ichikawa, F. Nakamura, M. Higasa, H. Akiyama, H. Nobumasa, S. Shiojima, et al. Ultrasensitive DNA Chip: Gene Expression Profile Analysis without RNA Amplification. J. Biochem., April 1, 2006; 139(4): 697 - 703. [Abstract] [Full Text] [PDF]

				Y. Xie, E.E. Puscheck, and D.A. Rappolee Effects of SAPK/JNK inhibitors on preimplantation mouse embryo development are influenced greatly by the amount of stress induced by the media Mol. Hum. Reprod., April 1, 2006; 12(4): 217 - 224. [Abstract] [Full Text] [PDF]

				L. J. Van Winkle, J. K. Tesch, A. Shah, and A. L. Campione System B0,+ amino acid transport regulates the penetration stage of blastocyst implantation with possible long-term developmental consequences through adulthood Hum. Reprod. Update, March 1, 2006; 12(2): 145 - 157. [Abstract] [Full Text] [PDF]