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First published online 19 April 2006
doi: 10.1242/dev.02372

Development 133, 1979-1989 (2006)
Published by The Company of Biologists 2006
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Senseless physically interacts with proneural proteins and functions as a transcriptional co-activator

Melih Acar1,*, Hamed Jafar-Nejad2,3,*, Nikolaos Giagtzoglou3, Sasidhar Yallampalli2, Gabriela David2, Yuchun He3, Christos Delidakis4 and Hugo J. Bellen1,2,3,{dagger}

1 Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.
2 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
3 Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.
4 Institute of Molecular Biology and Biotechnology, FORTH and Department of Biology, University of Crete, Heraklion, GR-71110, Greece.

{dagger} Author for correspondence (e-mail: hbellen{at}bcm.tmc.edu)

Accepted 20 March 2006

The zinc-finger transcription factor Senseless is co-expressed with basic helix-loop-helix (bHLH) proneural proteins in Drosophila sensory organ precursors and is required for their normal development. High levels of Senseless synergize with bHLH proteins and upregulate target gene expression, whereas low levels of Senseless act as a repressor in vivo. However, the molecular mechanism for this dual role is unknown. Here, we show that Senseless binds bHLH proneural proteins via its core zinc fingers and is recruited by proneural proteins to their target enhancers to function as a co-activator. Some point mutations in the Senseless zinc-finger region abolish its DNA-binding ability but partially spare the ability of Senseless to synergize with proneural proteins and to induce sensory organ formation in vivo. Therefore, we propose that the structural basis for the switch between the repressor and co-activator functions of Senseless is the ability of its core zinc fingers to interact physically with both DNA and bHLH proneural proteins. As Senseless zinc fingers are ~90% identical to the corresponding zinc fingers of its vertebrate homologue Gfi1, which is thought to cooperate with bHLH proteins in several contexts, the Senseless/bHLH interaction might be evolutionarily conserved.

Key words: Drosophila, Senseless


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