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First published online 13 September 2006
doi: 10.1242/dev.02583

Development 133, 3983-3992 (2006)
Published by The Company of Biologists 2006
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What's this?

Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain

Jason D. Berndt and Mary C. Halloran*

Departments of Zoology and Anatomy and Neuroscience Training Program, University of Wisconsin, Madison, WI 53706, USA.

* Author for correspondence (e-mail: mchalloran{at}wisc.edu)

Accepted 11 August 2006

Neural crest cells (NCCs) are pluripotent migratory cells that are crucial to the development of the peripheral nervous system, pigment cells and craniofacial cartilage and bone. NCCs are specified within the dorsal ectoderm and undergo an epithelial to mesenchymal transition (EMT) in order to migrate to target destinations where they differentiate. Here we report a role for a member of the semaphorin family of cell guidance molecules in NCC development. Morpholino-mediated knockdown of Sema3d inhibits the proliferation of hindbrain neuroepithelial cells. In addition, Sema3d knockdown reduces markers of migratory NCCs and disrupts NCC-derived tissues. Similarly, expression of a dominant-repressor form of TCF ({Delta}TCF) reduces hindbrain cell proliferation and leads to a disruption of migratory NCC markers. Moreover, expression of {Delta}TCF downregulates sema3d RNA expression. Finally, Sema3d overexpression rescues reduced proliferation caused by {Delta}TCF expression, suggesting that Sema3d lies downstream of Wnt/TCF signaling in the molecular pathway thought to control cell cycle in NCC precursors.

Key words: Neural crest, Zebrafish, Semaphorin, TCF, Cell cycle, Cyclin


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Related articles in Development:

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Development 2006 133: e2004. [Full Text]  



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[Abstract] [Full Text] [PDF]


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