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First published online 11 October 2006
doi: 10.1242/dev.02620

Development 133, 4475-4483 (2006)
Published by The Company of Biologists 2006
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DNA supercoiling factor contributes to dosage compensation in Drosophila

Hirofumi Furuhashi*, Mikage Nakajima and Susumu Hirose{dagger}

Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, SOKENDAI, Mishima, Shizuoka-ken 411-8540, Japan.

{dagger} Author for correspondence (e-mail: shirose{at}lab.nig.ac.jp)

Accepted 7 September 2006

DNA supercoiling factor (SCF) is a protein capable of generating negative supercoils in DNA in conjunction with topoisomerase II. To clarify the biological functions of SCF, we introduced a heritable SCF RNAi into Drosophila. Upon knockdown of SCF, we observed male lethality and male-specific reduction in the expression levels of X-linked genes. SCF functionally interacts with components of the MSL complex, which are required for dosage compensation via hypertranscription of the male X chromosome. Moreover, SCF colocalizes with the MSL complex along the male X chromosome. Upon overexpression of SCF, the male X chromosome had a bloated appearance. This phenotype was dependent on the histone acetyltransferase MOF and was suppressed by simultaneous overexpression of ISWI. These findings demonstrate that SCF plays a role in transcriptional activation via alteration of chromatin structure and provide evidence that SCF contributes to dosage compensation.

Key words: Supercoiling factor, Dosage compensation, Chromatin remodeling, MSL complex, Drosophila


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Development 2006 133: e2202. [Full Text]  



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