QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 8 February 2006
doi: 10.1242/dev.02269

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.02269v1 133/6/1013    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pouget, C. Articles by Jaffredo, T. Search for Related Content PubMed PubMed Citation Articles by Pouget, C. Articles by Jaffredo, T.

Somite-derived cells replace ventral aortic hemangioblasts and provide aortic smooth muscle cells of the trunk

¹ Université Pierre et Marie Curie-Paris6, CNRS UMR7622, Laboratoire de Biologie du Développement, Bat C, 6^ème étage, Case 24, 75252 Paris Cedex 05, France.
² UMR 7128 Laboratoire d'Embryologie Cellulaire et Moléculaire, 49 bis, avenue de la Belle Gabrielle, 94130 Nogent-sur-Marne, France.

^* Author for correspondence (e-mail: jaffredo{at}ccr.jussieu.fr)

Accepted 3 January 2006

We have previously shown that endothelial cells of the aortic floor give rise to hematopoietic cells, revealing the existence of an aortic hemangioblast. It has been proposed that the restriction of hematopoiesis to the aortic floor is based on the existence of two different and complementary endothelial lineages that form the vessel: one originating from the somite would contribute to the roof and sides, another from the splanchnopleura would contribute to the floor. Using quail/chick orthotopic transplantations of paraxial mesoderm, we have traced the distribution of somite-derived endothelial cells during aortic hematopoiesis. We show that the aortic endothelium undergoes two successive waves of remodeling by somitic cells: one when the aortae are still paired, during which the initial roof and sides of the vessels are renewed; and a second, associated to aortic hematopoiesis, in which the hemogenic floor is replaced by somite endothelial cells. This floor thus appears as a temporary structure, spent out and replaced. In addition, the somite contributes to smooth muscle cells of the aorta. In vivo lineage tracing experiments with non-replicative retroviral vectors showed that endothelial cells do not give rise to smooth muscle cells. However, in vitro, purified endothelial cells acquire smooth muscle cells characteristics. Taken together, these data point to the crucial role of the somite in shaping the aorta and also give an explanation for the short life of aortic hematopoiesis.

Key words: Avian embryo, Aorta, Hemangioblasts, Hematopoiesis, Endothelium, Somite, Vascular smooth muscle

This article has been cited by other articles:

				P. Wasteson, B. R. Johansson, T. Jukkola, S. Breuer, L. M. Akyurek, J. Partanen, and P. Lindahl Developmental origin of smooth muscle cells in the descending aorta in mice Development, May 15, 2008; 135(10): 1823 - 1832. [Abstract] [Full Text] [PDF]

				R. Ben-Yair and C. Kalcheim Notch and bone morphogenetic protein differentially act on dermomyotome cells to generate endothelium, smooth, and striated muscle J. Cell Biol., February 6, 2008; 180(3): 607 - 618. [Abstract] [Full Text] [PDF]

				S. Tozer, M.-A. Bonnin, F. Relaix, S. Di Savino, P. Garcia-Villalba, P. Coumailleau, and D. Duprez Involvement of vessels and PDGFB in muscle splitting during chick limb development Development, July 15, 2007; 134(14): 2579 - 2591. [Abstract] [Full Text] [PDF]

				M. W. Majesky Developmental Basis of Vascular Smooth Muscle Diversity Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1248 - 1258. [Abstract] [Full Text] [PDF]