|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 15 February 2006
doi: 10.1242/dev.02279
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Cardiovascular Research Center and Cardiology Division, Massachusetts General
Hospital and Harvard Medical School, Boston, MA, USA.
2 Novartis Institutes for Biomedical Research, 250 Massachusetts Avenue,
Cambridge, MA, USA.
* Author for correspondence (e-mail: dmilan{at}partners.org)
Accepted 10 January 2006
Normal heart function is critically dependent on the timing and coordination provided by a complex network of specialized cells: the cardiac conduction system. We have employed functional assays in zebrafish to explore early steps in the patterning of the conduction system that previously have been inaccessible. We demonstrate that a ring of atrioventricular conduction tissue develops at 40 hours post-fertilization in the zebrafish heart. Analysis of the mutant cloche reveals a requirement for endocardial signals in the formation of this tissue. The differentiation of these specialized cells, unlike that of adjacent endocardial cushions and valves, is not dependent on blood flow or cardiac contraction. Finally, both neuregulin and notch1b are necessary for the development of atrioventricular conduction tissue. These results are the first demonstration of the endocardial signals required for patterning central `slow' conduction tissue, and they reveal the operation of distinct local endocardial-myocardial interactions within the developing heart tube.
Key words: Notch, Neuregulin, Cardiac conduction system, Development, Zebrafish
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  V. Mehta, R. E. Peterson, and W. Heideman 2,3,7,8-Tetrachlorodibenzo-p-dioxin Exposure Prevents Cardiac Valve Formation in Developing Zebrafish Toxicol. Sci., August 1, 2008; 104(2): 303 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Honjo, J. Kniss, and J. S. Eisen Neuregulin-mediated ErbB3 signaling is required for formation of zebrafish dorsal root ganglion neurons Development, August 1, 2008; 135(15): 2615 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Chi, R. M. Shaw, S. De Val, G. Kang, L. Y. Jan, B. L. Black, and D. Y.R. Stainier Foxn4 directly regulates tbx2b expression and atrioventricular canal formation Genes & Dev., March 15, 2008; 22(6): 734 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Scherz, J. Huisken, P. Sahai-Hernandez, and D. Y. R. Stainier High-speed imaging of developing heart valves reveals interplay of morphogenesis and function Development, March 15, 2008; 135(6): 1179 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Arnaout, T. Ferrer, J. Huisken, K. Spitzer, D. Y. R. Stainier, M. Tristani-Firouzi, and N. C. Chi Zebrafish model for human long QT syndrome PNAS, July 3, 2007; 104(27): 11316 - 11321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Holtzman, J. J. Schoenebeck, H.-J. Tsai, and D. Yelon Endocardium is necessary for cardiomyocyte movement during heart tube assembly Development, June 15, 2007; 134(12): 2379 - 2386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Leslie, L. Ariza-McNaughton, A. L. Bermange, R. McAdow, S. L. Johnson, and J. Lewis Endothelial signalling by the Notch ligand Delta-like 4 restricts angiogenesis Development, March 1, 2007; 134(5): 839 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Mably, L. P. Chuang, F. C. Serluca, M.-A. P. K. Mohideen, J.-N. Chen, and M. C. Fishman santa and valentine pattern concentric growth of cardiac myocardium in the zebrafish Development, August 15, 2006; 133(16): 3139 - 3146. [Abstract] [Full Text] [PDF]
|
|
