|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 13 June 2007
doi: 10.1242/dev.005611
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,
RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,
Japan.
2 Laboratory of Molecular Cell Biology and Development, Graduate School of
Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto
606-8502, Japan.
3 Department of Biosystems Science, Graduate School of Science and Technology,
Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
4 Cell Resource Center for Biomedical Research, Institute of Development, Aging
and Cancer, Tohoku University, 4-1 Seiryo-cho, Aoba-ku, Sendai 980-8575,
Japan.
5 Department of Genetics and Division of Mammalian Development, National
Institute of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411-8540,
Japan.
6 Department of Cell Biology, Institute for Virus Research, Kyoto University,
Shogoin Kawara-cho, Kyoto 606-8507, Japan.
7 Precursory Research for Embryonic Science and Technology, Japan Science and
Technology Agency, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
* Author for correspondence (e-mail: saitou{at}cdb.riken.jp)
Accepted 10 May 2007
We previously reported that primordial germ cells (PGCs) in mice erase genome-wide DNA methylation and histone H3 lysine9 dimethylation (H3K9me2), and instead acquire high levels of tri-methylation of H3K27 (H3K27me3) during their migration, a process that might be crucial for the re-establishment of potential totipotency in the germline. We here explored a cellular dynamics associated with this epigenetic reprogramming. We found that PGCs undergo erasure of H3K9me2 and upregulation of H3K27me3 in a progressive, cell-by-cell manner, presumably depending on their developmental maturation. Before or concomitant with the onset of H3K9 demethylation, PGCs entered the G2 arrest of the cell cycle, which apparently persisted until they acquired high H3K27me3 levels. Interestingly, PGCs exhibited repression of RNA polymerase II-dependent transcription, which began after the onset of H3K9me2 reduction in the G2 phase and tapered off after the acquisition of high-level H3K27me3. The epigenetic reprogramming and transcriptional quiescence were independent from the function of Nanos3. We found that before H3K9 demethylation, PGCs exclusively repress an essential histone methyltransferase, GLP, without specifically upregulating histone demethylases. We suggest the possibility that active repression of an essential enzyme and subsequent unique cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs.
Key words: Primordial germ cells (PGCs), Histone modifications, Reprogramming, Cell cycle, Epigenetics, Mouse
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Hayashi and M. A. Surani Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro Development, November 1, 2009; 136(21): 3549 - 3556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Edson, A. K. Nagaraja, and M. M. Matzuk The Mammalian Ovary from Genesis to Revelation Endocr. Rev., October 1, 2009; 30(6): 624 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yoshioka, J. R. McCarrey, and Y. Yamazaki Dynamic Nuclear Organization of Constitutive Heterochromatin During Fetal Male Germ Cell Development in Mice Biol Reprod, April 1, 2009; 80(4): 804 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nakamura and G. Seydoux Less is more: specification of the germline by transcriptional repression Development, December 1, 2008; 135(23): 3817 - 3827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J Ross, N. P Ragina, R. M Rodriguez, A. E Iager, K. Siripattarapravat, N. Lopez-Corrales, and J. B Cibelli Polycomb gene expression and histone H3 lysine 27 trimethylation changes during bovine preimplantation development Reproduction, December 1, 2008; 136(6): 777 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.A. Surani, G. Durcova-Hills, P. Hajkova, K. Hayashi, and W.W. Tee Germ Line, Stem Cells, and Epigenetic Reprogramming Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.015v1. [Abstract] [PDF]
|
|
|
|
|
|
Y. Ohinata, M. Sano, M. Shigeta, K. Yamanaka, and M. Saitou A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter Reproduction, October 1, 2008; 136(4): 503 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mise, T. Fuchikami, M. Sugimoto, S. Kobayakawa, F. Ike, T. Ogawa, T. Tada, S. Kanaya, T. Noce, and K. Abe Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells, August 1, 2008; 13(8): 863 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurimoto, Y. Yabuta, Y. Ohinata, M. Shigeta, K. Yamanaka, and M. Saitou Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice Genes & Dev., June 15, 2008; 22(12): 1617 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ghosh and G. Seydoux Inhibition of Transcription by the Caenorhabditis elegans Germline Protein PIE-1: Genetic Evidence for Distinct Mechanisms Targeting Initiation and Elongation Genetics, January 1, 2008; 178(1): 235 - 243. [Abstract] [Full Text] [PDF]
|
|
