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First published online 13 December 2006
doi: 10.1242/dev.02728

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Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis

Nikolay Ninov, Dominic A. Chiarelli^* and Enrique Martín-Blanco

Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Cientific de Barcelona, Josep Samitier 1-5, Barcelona 08028, Spain.

Author for correspondence (e-mail: embbmc{at}cid.csic.es)

Accepted 2 November 2006

The fusion of epithelial sheets is an essential morphogenetic event. Here, we study the development of the abdomen of Drosophila as a model of bounded epithelia expansion and uncover a complex multistep process for the generation of the adult epidermis from histoblasts, founder cells that replace the larval cells during metamorphosis. We find that histoblasts experience a biphasic cell cycle and emit apical projections that direct their invasive planar intercalation in between larval cells. Coordinately, the larval cells extrude from the epithelia by apical constriction of an actomyosin ring and as a consequence die by apoptosis and are removed by circulating haemocytes. We demonstrate that the proliferation of histoblasts and the death of larval cells are triggered by two independent extrinsic Ecdysone hormonal pulses. Finally, we show that histoblast spreading and the death of larval cells depend on a mutual exchange of signals and are non-autonomous processes.

Key words: Drosophila, Metamorphosis, Histoblasts, Morphogenesis, Abdomen, Cell death, Cell replacement

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