QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 3 October 2007
doi: 10.1242/dev.009654

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.009654v1 134/21/3849    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furutani, M. Articles by Tasaka, M. Search for Related Content PubMed PubMed Citation Articles by Furutani, M. Articles by Tasaka, M. Social Bookmarking                         What's this?

The gene MACCHI-BOU 4/ENHANCER OF PINOID encodes a NPH3-like protein and reveals similarities between organogenesis and phototropism at the molecular level

Masahiko Furutani^1,*, Takahito Kajiwara^1,*, Takehide Kato¹, Birgit S. Treml², Christine Stockum², Ramón A. Torres-Ruiz² and Masao Tasaka^1,

¹ Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara 630-0101, Japan.
² Lehrstuhl für Genetik, Technische Universität München, Wissenschaftszentrum Weihenstephan, Am Hochanger 8, 85350 Freising, Germany.

Author for correspondence (e-mail: m-tasaka{at}bs.naist.jp)

Accepted 10 August 2007

Intercellular transport of the phytohormone auxin is a significant factor for plant organogenesis. To investigate molecular mechanisms by which auxin controls organogenesis, we analyzed the macchi-bou 4 (mab4) mutant identified as an enhancer of pinoid (pid). Although mab4 and pid single mutants displayed relatively mild cotyledon phenotypes, pid mab4 double mutants completely lacked cotyledons. We found that MAB4 was identical to ENHANCER OF PINOID (ENP), which has been suggested to control PIN1 polarity in cotyledon primordia. MAB4/ENP encodes a novel protein, which belongs to the NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) family thought to function as a signal transducer in phototropism and control lateral translocation of auxin. MAB4/ENP mRNA was detected in the protodermal cell layer of the embryo and the meristem L1 layer at the site of organ initiation. In the mab4 embryo, the abundance of PIN1:GFP was severely decreased at the plasma membrane in the protodermal cell layer. In addition, subcellular localization analyses indicated that MAB4/ENP resides on a subpopulation of endosomes as well as on unidentified intracellular compartments. These results indicate that MAB4/ENP is involved in polar auxin transport in organogenesis.

Key words: Polar auxin transport, Organ development, PIN1, PID, NPH3, Arabidopsis thaliana

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R. J. Jurkuta, N. J. Kaplinsky, J. E. Spindel, and M. K. Barton Partitioning the Apical Domain of the Arabidopsis Embryo Requires the BOBBER1 NudC Domain Protein PLANT CELL, July 1, 2009; 21(7): 1957 - 1971. [Abstract] [Full Text] [PDF]

				J. J. Holland, D. Roberts, and E. Liscum Understanding phototropism: from Darwin to today J. Exp. Bot., May 1, 2009; 60(7): 1969 - 1978. [Abstract] [Full Text] [PDF]

				Y. Cheng, G. Qin, X. Dai, and Y. Zhao NPY genes and AGC kinases define two key steps in auxin-mediated organogenesis in Arabidopsis PNAS, December 30, 2008; 105(52): 21017 - 21022. [Abstract] [Full Text] [PDF]