QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 14 November 2007
doi: 10.1242/dev.008151

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: dev.008151v1 134/24/4315    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohta, S. Articles by Yamada, G. Search for Related Content PubMed PubMed Citation Articles by Ohta, S. Articles by Yamada, G. Social Bookmarking                         What's this?

Cessation of gastrulation is mediated by suppression of epithelial-mesenchymal transition at the ventral ectodermal ridge

¹ Center for Animal Resources and Development (CARD), Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811, Japan.
² JSPS research fellow, Chiyoda-ku 1-8, Tokyo 102-8472, Japan.
³ Department of Anatomy, Fukuoka University School of Medicine, Fukuoka, Japan.
⁴ Department of Developmental Neurobiology Graduate School of Medical Sciences, Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811, Japan.

^* Author for correspondence (e-mail: gensan{at}gpo.kumamoto-u.ac.jp)

Accepted 5 September 2007

In the gastrula stage embryo, the epiblast migrates toward the primitive streak and ingresses through the primitive groove. Subsequently, the ingressing epiblast cells undergo epithelial-mesenchymal transition (EMT) and differentiate into the definitive endoderm and mesoderm during gastrulation. However, the developmental mechanisms at the end of gastrulation have not yet been elucidated. Histological and genetic analyses of the ventral ectodermal ridge (VER), a derivative of the primitive streak, were performed using chick and mouse embryos. The analyses showed a continued cell movement resembling gastrulation associated with EMT during the early tailbud stage of both embryos. Such gastrulation-like cell movement was gradually attenuated by the absence of EMT during tail development. The kinetics of the expression pattern of noggin (Nog) and basal membrane degradation adjacent to the chick and the mouse VER indicated a correlation between the temporal and/or spatial expression of Nog and the presence of EMT in the VER. Furthermore, Nog overexpression suppressed EMT and arrested ingressive cell movement in the chick VER. Mice mutant in noggin displayed dysregulation of EMT with continued ingressive cell movement. These indicate that the inhibition of Bmp signaling by temporal and/or spatial Nog expression suppresses EMT and leads to the cessation of the ingressive cell movement from the VER at the end of gastrulation.

Key words: Chick, Mouse, Bmp, Noggin, Gastrulation, Epithelial-mesenchymal transition (EMT), Ventral ectodermal ridge (VER), Tail, Sonoporation

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				V. Wilson, I. Olivera-Martinez, and K. G. Storey Stem cells, signals and vertebrate body axis extension Development, May 15, 2009; 136(10): 1591 - 1604. [Abstract] [Full Text] [PDF]

				M. J. McGrew, A. Sherman, S. G. Lillico, F. M. Ellard, P. A. Radcliffe, H. J. Gilhooley, K. A. Mitrophanous, N. Cambray, V. Wilson, and H. Sang Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity Development, July 1, 2008; 135(13): 2289 - 2299. [Abstract] [Full Text] [PDF]