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First published online 24 July 2008
doi: 10.1242/dev.023648

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Vascular signalling mediated by ZWILLE potentiates WUSCHEL function during shoot meristem stem cell development in the Arabidopsis embryo

¹ Faculty of Biology, Schaenzlestrasse 1, University of Freiburg, D-79104 Freiburg, Germany.
² Developmental Genetics, Centre for Molecular Biology of Plants, University of Tübingen, D-72076 Tübingen, Germany.

^* Author for correspondence (e-mail: laux{at}biologie.uni-freiburg.de)

Accepted 4 July 2008

SUMMARY

Stem cells are maintained in an undifferentiated state by signals from their microenvironment, the stem cell niche. Despite its central role for organogenesis throughout the plant's life, little is known about how niche development is regulated in the Arabidopsis embryo. Here we show that, in the absence of functional ZWILLE (ZLL), which is a member of the ARGONAUTE (AGO) family, stem cell-specific expression of the signal peptide gene CLAVATA3 (CLV3) is not maintained despite increased levels of the homeodomain transcription factor WUSCHEL (WUS), which is expressed in the organising centre (OC) of the niche and normally promotes stem cell identity. Tissue-specific expression indicates that ZLL acts to maintain the stem cells from the neighbouring vascular primordium, providing direct evidence for a non-cell-autonomous mechanism. Furthermore, mutant and marker gene analyses suggest that during shoot meristem formation, ZLL functions in a similar manner but in a sequential order with its close homologue AGO1, which mediates RNA interference. Thus, WUS-dependent OC signalling to the stem cells is promoted by AGO1 and subsequently maintained by a provascular ZLL-dependent signalling pathway.

Key words: Arabidopsis embryogenesis, Meristem, Stem cell niche, ZWILLE (PINHEAD; AGO10)

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