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First published online 24 July 2008
doi: 10.1242/dev.022194

Development 135, 2895-2904 (2008)
Published by The Company of Biologists 2008
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Self-modulation of Notch signaling during ommatidial development via the Roughened eye transcriptional repressor

David del Alamo* and Marek Mlodzik{dagger}

Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, USA.

{dagger} Author for correspondence (e-mail: marek.mlodzik{at}mssm.edu)

Accepted 15 June 2008

The Notch (N) signaling pathway is involved in a vast number of patterning processes in all metazoans. The regulation of the core N pathway is largely understood, but little is known about fine-tuning modulatory effects. Here, we address the role of Drosophila Krüppel-family Zn-finger transcription factor roughened eye (roe) in the context of N signaling. We demonstrate that during eye patterning, N signaling regulates the expression of roe. In turn, Roe negatively modulates the expression of target genes of N-signaling activation. In the absence of roe function, expression of N target genes is elevated and the resulting phenotypes during patterning of the retina are similar to those of N gain-of-function scenarios. Importantly, our data show that Roe binds regulatory DNA sequences of N target genes of the E(spl)-complex both in vitro and in vivo, independently of Su(H)-DNA interaction. Thus, our data suggest that Roe acts as a transcriptional repressor in a negative-feedback loop of the N pathway.

Key words: Drosophila, Cell signaling, Feedback modulation, Transcriptional repression


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© The Company of Biologists Ltd 2008