QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 17 September 2008
doi: 10.1242/dev.025916

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.025916v1 135/20/3447    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cheng, X. Articles by Keller, G. M. Search for Related Content PubMed PubMed Citation Articles by Cheng, X. Articles by Keller, G. M. Social Bookmarking                         What's this?

Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast

¹ Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
² Stem Cell and Developmental Biology Department, Genome Institute of Singapore, 138672 Singapore.
³ McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada.

^* Author for correspondence (e-mail: gkeller{at}uhnresearch.ca)

Accepted 1 September 2008

During embryonic development, the establishment of the primitive erythroid lineage in the yolk sac is a temporally and spatially restricted program that defines the onset of hematopoiesis. In this report, we have used the embryonic stem cell differentiation system to investigate the regulation of primitive erythroid development at the level of the hemangioblast. We show that the combination of Wnt signaling with inhibition of the Notch pathway is required for the development of this lineage. Inhibition of Notch signaling at this stage appears to be mediated by the transient expression of Numb in the hemangioblast-derived blast cell colonies. Activation of the Notch pathway was found to inhibit primitive erythropoiesis efficiently through the upregulation of inhibitors of the Wnt pathway. Together, these findings demonstrate that specification of the primitive erythroid lineage is controlled, in part, by the coordinated interaction of the Wnt and Notch pathways, and position Numb as a key mediator of this process.

Key words: Notch signaling, Numb, Wnt signaling, Hemangioblast, Primitive erythropoiesis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in Development:

Dishing up blood from ES cells

Development 2008 135: e2004. [Full Text]  

This article has been cited by other articles:

				S. Irion, M.C. Nostro, S.J. Kattman, and G.M. Keller Directed Differentiation of Pluripotent Stem Cells: From Developmental Biology to Therapeutic Applications Cold Spring Harb Symp Quant Biol, March 27, 2009; (2009) sqb.2008.73.065v1. [Abstract] [PDF]

				M. Shin, H. Nagai, and G. Sheng Notch mediates Wnt and BMP signals in the early separation of smooth muscle progenitors and blood/endothelial common progenitors Development, February 15, 2009; 136(4): 595 - 603. [Abstract] [Full Text] [PDF]