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First published online 16 October 2008
doi: 10.1242/dev.025114

Development 135, 3697-3706 (2008)
Published by The Company of Biologists 2008
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{alpha}-Endosulfine is a conserved protein required for oocyte meiotic maturation in Drosophila

Jessica R. Von Stetina1, Susanne Tranguch1, Sudhansu K. Dey1,2,3, Laura A. Lee1, Byeong Cha1 and Daniela Drummond-Barbosa1,*

1 Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
2 Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
3 Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

* Author for correspondence (e-mail: daniela.drummond-barbosa{at}vanderbilt.edu)

Accepted 16 September 2008

Meiosis is coupled to gamete development and must be well regulated to prevent aneuploidy. During meiotic maturation, Drosophila oocytes progress from prophase I to metaphase I. The molecular factors controlling meiotic maturation timing, however, are poorly understood. We show that Drosophila {alpha}-endosulfine (endos) plays a key role in this process. endos mutant oocytes have a prolonged prophase I and fail to progress to metaphase I. This phenotype is similar to that of mutants of cdc2 (synonymous with cdk1) and of twine, the meiotic homolog of cdc25, which is required for Cdk1 activation. We found that Twine and Polo kinase levels are reduced in endos mutants, and identified Early girl (Elgi), a predicted E3 ubiquitin ligase, as a strong Endos-binding protein. In elgi mutant oocytes, the transition into metaphase I occurs prematurely, but Polo and Twine levels are unaffected. These results suggest that Endos controls meiotic maturation by regulating Twine and Polo levels, and, independently, by antagonizing Elgi. Finally, germline-specific expression of the human {alpha}-endosulfine ENSA rescues the endos mutant meiotic defects and infertility, and {alpha}-endosulfine is expressed in mouse oocytes, suggesting potential conservation of its meiotic function.

Key words: Meiosis, Oogenesis, {alpha}-Endosulfine, Cdc25, Polo, E3 ubiquitin ligase, Drosophila


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This article has been cited by other articles:


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[Abstract] [Full Text] [PDF]


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