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First published online 23 October 2008
doi: 10.1242/dev.029009

This Article Figures Only Full Text Full Text (PDF) Supplementary Material A corrigendum has been published All Versions of this Article: dev.029009v1 135/23/3829    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gervais, L. Articles by Guichet, A. Search for Related Content PubMed PubMed Citation Articles by Gervais, L. Articles by Guichet, A. Social Bookmarking                         What's this?

PIP5K-dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte

¹ Institut Jacques Monod, Unité Mixte de Recherche 7592, CNRS, Universités Paris 7, 2 place Jussieu, F-75251, Paris Cedex 05, France.
² Cell Division Group, ICREA and IRB, Parc Cientific de Barcelona, c/Baldiri Reixac 10-12, 08028 Barcelona, Spain.
³ Institut für Entwicklungsbiologie, Universität zu Köln, Gyrhofstrasse 17, 50923, Köln, Germany.

^* Author for correspondence (e-mail: guichet{at}ijm.jussieu.fr)

Accepted 28 September 2008

The attachment of the cytoskeleton to the plasma membrane is crucial in controlling the polarized transport of cell-fate-determining molecules. Attachment involves adaptor molecules, which have the capacity to bind to both the plasma membrane and elements of the cytoskeleton, such as microtubules and actin filaments. Using the Drosophila oocyte as a model system, we show that the type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K), Skittles, is necessary to sustain the organization of microtubules and actin cytoskeleton required for the asymmetric transport of oskar, bicoid and gurken mRNAs and thereby controls the establishment of cell polarity. We show that Skittles function is crucial to synthesize and maintain phosphatidylinositol 4,5 bisphosphate (PIP2) at the plasma membrane in the oocyte. Reduction of Skittles activity impairs activation at the plasma membrane of Moesin, a member of the ERM family known to link the plasma membrane to the actin-based cytoskeleton. Furthermore, we provide evidence that Skittles, by controlling the localization of Bazooka, Par-1 and Lgl, but not Lkb1, to the cell membrane, regulates PAR polarity proteins and the maintenance of specific cortical domains along the anteroposterior axis.

Key words: Drosophila, Microtubules, PAR proteins, PIP2, PIP5K, Polarity

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