QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 19 December 2007
doi: 10.1242/dev.016253

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.016253v1 135/3/463    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tian, A.-G. Articles by Deng, W.-M. Search for Related Content PubMed PubMed Citation Articles by Tian, A.-G. Articles by Deng, W.-M. Social Bookmarking                         What's this?

Lgl and its phosphorylation by aPKC regulate oocyte polarity formation in Drosophila

Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370, USA.

^* Author for correspondence (e-mail: wumin{at}bio.fsu.edu)

Accepted 6 November 2007

Specification of the anteroposterior (AP) axis in Drosophila oocytes requires proper organization of the microtubule and actin cytoskeleton. The establishment and regulation of cytoskeletal polarity remain poorly understood, however. Here, we show important roles for the tumor suppressor Lethal (2) giant larvae (Lgl) and atypical protein kinase C (aPKC) in regulating microtubule polarity and setting up the AP axis of the oocyte. Lgl in the germline cells regulates the localization of axis-specifying morphogens. aPKC phosphorylation of Lgl restricts Lgl activity to the oocyte posterior, thereby dividing the cortex into different domains along the AP axis. Active Lgl promotes the formation of actin-rich projections at the oocyte cortex and the posterior enrichment of the serine/threonine kinase Par-1, a key step for oocyte polarization. Our studies suggest that Lgl and its phosphorylation by aPKC may form a conserved regulatory circuitry in polarization of various cell types.

Key words: Lethal (2) giant larvae (Lgl), Atypical protein kinase C (aPKC), Oocyte polarity, Par-1, Microtubule, Oogenesis, Drosophila

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in Development:

Par-sing the regulatory circuitry of oocyte polarity

Development 2008 135: e302. [Full Text]  

This article has been cited by other articles:

				S. Kim, I. Gailite, B. Moussian, S. Luschnig, M. Goette, K. Fricke, M. Honemann-Capito, H. Grubmuller, and A. Wodarz Kinase-activity-independent functions of atypical protein kinase C in Drosophila J. Cell Sci., October 15, 2009; 122(20): 3759 - 3771. [Abstract] [Full Text] [PDF]

				S. Roth and J. A. Lynch Symmetry Breaking During Drosophila Oogenesis Cold Spring Harb Perspect Biol, August 1, 2009; 1(2): a001891 - a001891. [Abstract] [Full Text] [PDF]

				M. Jansen, J. P. ten Klooster, G. J. Offerhaus, and H. Clevers LKB1 and AMPK Family Signaling: The Intimate Link Between Cell Polarity and Energy Metabolism Physiol Rev, July 1, 2009; 89(3): 777 - 798. [Abstract] [Full Text] [PDF]

				M. Masuda-Hirata, A. Suzuki, Y. Amano, K. Yamashita, M. Ide, T. Yamanaka, M. Sakai, M. Imamura, and S. Ohno Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex Genes Cells, July 1, 2009; 14(7): 835 - 850. [Abstract] [Full Text] [PDF]

				L. Gervais, S. Claret, J. Januschke, S. Roth, and A. Guichet PIP5K-dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte Development, December 1, 2008; 135(23): 3829 - 3838. [Abstract] [Full Text] [PDF]

				C. Chabu and C. Q. Doe Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression Development, August 15, 2008; 135(16): 2739 - 2746. [Abstract] [Full Text] [PDF]

				Development in press Par-sing the regulatory circuitry of oocyte polarity J. Cell Sci., February 1, 2008; 121(3): e308 - e308. [Full Text] [PDF]