spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 4 Jul 2007
doi: 10.1242/dev.003764

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.003764v1
134/15/2863    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baker, C. C.
Right arrow Articles by Fuller, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baker, C. C.
Right arrow Articles by Fuller, M. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog


Catherine C. Baker and Margaret T. Fuller*
* Author for correspondence (e-mail: fuller{at}cmgm.stanford.edu)

Translational control is crucial for proper timing of developmental events that take place in the absence of transcription, as in meiotic activation in oocytes, early embryogenesis in many organisms, and spermatogenesis. Here we show that a novel form of the translation initiation complex component eIF4G in Drosophila, eIF4G2, is required specifically for male germ cells to undergo meiotic division and proper spermatid differentiation. Flies mutant for eIF4G2 are viable and female fertile but male sterile. Spermatocytes form, but the germ cells in mutant males skip the major events of the meiotic divisions and form aberrant spermatids with large nuclei. Consistent with the failure to undergo the meiotic divisions, function of eIF4G2 is required post-transcriptionally for normal accumulation of the core cell cycle regulatory proteins Twine and CycB in mature spermatocytes. Loss of eIF4G2 function also causes widespread defects in spermatid differentiation. Although differentiation markers Dj and Fzo are expressed in late-stage eIF4G2 mutant germ cells, several key steps of spermatid differentiation fail, including formation of a compact mitochondrial derivative and full elongation. Our results suggest that an alternate form of the translation initiation machinery may be required for regulation and execution of key steps in male germ cell differentiation.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. A. Henderson, E. Cronland, S. Dunkelbarger, V. Contreras, S. Strome, and B. D. Keiper
A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm
J. Cell Sci., May 15, 2009; 122(10): 1529 - 1539.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
E.L. Davies and M.T. Fuller
Regulation of Self-renewal and Differentiation in Adult Stem Cell Lineages: Lessons from the Drosophila Male Germ Line
Cold Spring Harb Symp Quant Biol, March 27, 2009; (2009) sqb.2008.73.063v1.
[Abstract] [PDF]

Home page
 GeneticsHome page
L. M. Mikhaylova, K. Nguyen, and D. I. Nurminsky
Analysis of the Drosophila melanogaster Testes Transcriptome Reveals Coordinate Regulation of Paralogous Genes
Genetics, May 1, 2008; 179(1): 305 - 315.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Ramirez-Valle, S. Braunstein, J. Zavadil, S. C. Formenti, and R. J. Schneider
eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy
J. Cell Biol., April 21, 2008; 181(2): 293 - 307.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman, and S. DiNardo
A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes
Development, August 1, 2007; 134(15): 2851 - 2861.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007