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Development ePress online publication date 13 Aug 2003
doi: 10.1242/dev.00679
Research article
neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions
Takeshi Sasamura,
Nobuo Sasaki,
Fumiyasu Miyashita,
Shiho Nakao,
Hiroyuki O. Ishikawa,
Mikiko Ito,
Motoo Kitagawa,
Kenichi Harigaya,
Eric Spana,
David Bilder,
Norbert Perrimon,
and
Kenji Matsuno*
* Author for correspondence (e-mail: matsuno{at}rs.noda.tus.ac.jp)
Notch signalling, which is highly conserved from nematodes to mammals, plays crucial roles in many developmental processes. In the Drosophila embryo, deficiency in Notch signalling results in neural hyperplasia, commonly referred to as the neurogenic phenotype. We identify a novel maternal neurogenic gene, neurotic, and show that it is essential for Notch signalling. neurotic encodes a Drosophila homolog of mammalian GDP-fucose protein O-fucosyltransferase, which adds fucose sugar to epidermal growth factor-like repeats and is known to play a crucial role in Notch signalling. neurotic functions in a cell-autonomous manner, and genetic epistasis tests reveal that Neurotic is required for the activity of the full-length but not an activated form of Notch. Further, we show that neurotic is required for Fringe activity, which encodes a fucose-specific 
1,3 N-acetylglucosaminyltransferase, previously shown to modulate Notch receptor activity. Finally, Neurotic is essential for the physical interaction of Notch with its ligand Delta, and for the ability of Fringe to modulate this interaction in Drosophila cultured cells. We present an unprecedented example of an absolute requirement of a protein glycosylation event for a ligand-receptor interaction. Our results suggest that O-fucosylation catalysed by Neurotic is also involved in the Fringe-independent activities of Notch and may provide a novel on-off mechanism that regulates ligand-receptor interactions.
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|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
|
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Highly Conserved O-Fucose Sites Have Distinct Effects on Notch1 Function
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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130(26):
6411 - 6421.
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[Full Text]
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|
|
|
© The Company of Biologists Ltd 2003
