spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 20 Aug 2003
doi: 10.1242/dev.00706

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00706v1
130/20/4919    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galli, A.
Right arrow Articles by Dono, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galli, A.
Right arrow Articles by Dono, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research article

Glypican 4 modulates FGF signalling and regulates dorsoventral forebrain patterning in Xenopus embryos


Antonella Galli, Agnes Roure, Rolf Zeller, and Rosanna Dono*
* Author for correspondence (e-mail: r.dono{at}bio.uu.nl)

Heparan sulphate proteoglycans such as glypicans are essential modulators of intercellular communication during embryogenesis. In Xenopus laevis embryos, the temporal and spatial distribution of Glypican 4 (Gpc4) transcripts during gastrulation and neurulation suggests functions in early development of the central nervous system. We have functionally analysed the role of Xenopus Gpc4 by using antisense morpholino oligonucleotides and show that Gpc4 is part of the signalling network that patterns the forebrain. Depletion of GPC4 protein results in a pleiotropic phenotype affecting both primary axis formation and early patterning of the anterior central nervous system. Molecular analysis shows that posterior axis elongation during gastrulation is affected in GPC4-depleted embryos, whereas head and neural induction are apparently normal. During neurulation, loss of GPC4 disrupts expression of dorsal forebrain genes, such as Emx2, whereas genes marking the ventral forebrain and posterior central nervous system continue to be expressed. This loss of GPC4 activity also causes apoptosis of forebrain progenitors during neural tube closure. Biochemical studies establish that GPC4 binds FGF2 and modulates FGF signal transduction. Inhibition of FGF signal transduction, by adding the chemical SU5402 to embryos from neural plate stages onwards, phenocopies the loss of gene expression and apoptosis in the forebrain. We propose that GPC4 regulates dorsoventral forebrain patterning by positive modulation of FGF signalling.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. E. Bertolesi, G. Michaiel, and S. McFarlane
Two Heparanase Splicing Variants with Distinct Properties Are Necessary in Early Xenopus Development
J. Biol. Chem., June 6, 2008; 283(23): 16004 - 16016.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. Caneparo, Y.-L. Huang, N. Staudt, M. Tada, R. Ahrendt, O. Kazanskaya, C. Niehrs, and C. Houart
Dickkopf-1 regulates gastrulation movements by coordinated modulation of Wnt/betacatenin and Wnt/PCP activities, through interaction with the Dally-like homolog Knypek
Genes & Dev., February 15, 2007; 21(4): 465 - 480.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. J. Kim, I-H. Liu, Y. Song, J.-A. Lee, W. Halfter, R. J. Balice-Gordon, E. Linney, and G. J. Cole
Agrin is required for posterior development and motor axon outgrowth and branching in embryonic zebrafish
Glycobiology, February 1, 2007; 17(2): 231 - 247.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Williamson, J. Selfe, T. Gordon, Y.-J. Lu, K. Pritchard-Jones, K. Murai, P. Jones, P. Workman, and J. Shipley
Role for Amplification and Expression of Glypican-5 in Rhabdomyosarcoma
Cancer Res., January 1, 2007; 67(1): 57 - 65.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Vigetti, M. Ori, M. Viola, A. Genasetti, E. Karousou, M. Rizzi, F. Pallotti, I. Nardi, V. C. Hascall, G. De Luca, et al.
Molecular Cloning and Characterization of UDP-glucose Dehydrogenase from the Amphibian Xenopus laevis and Its Involvement in Hyaluronan Synthesis
J. Biol. Chem., March 24, 2006; 281(12): 8254 - 8263.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Ding, M. Lopez-Burks, J. A. Sanchez-Duran, M. Korc, and A. D. Lander
Growth factor-induced shedding of syndecan-1 confers glypican-1 dependence on mitogenic responses of cancer cells
J. Cell Biol., November 21, 2005; 171(4): 729 - 738.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
X. Lin
Functions of heparan sulfate proteoglycans in cell signaling during development
Development, December 15, 2004; 131(24): 6009 - 6021.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003