spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
Development ePress online publication date 22 Oct 2003
doi: 10.1242/dev.00840

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.00840v1
130/24/6013    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garcia-Dominguez, M.
Right arrow Articles by Charnay, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garcia-Dominguez, M.
Right arrow Articles by Charnay, P.

Research article

Ebf gene function is required for coupling neuronal differentiation and cell cycle exit


Mario Garcia-Dominguez, Christophe Poquet, Sonia Garel, and Patrick Charnay*
* Author for correspondence (e-mail: charnay{at}wotan.ens.fr)

Helix-loop-helix transcription factors of the Ebf/Olf1 family have previously been implicated in the control of neurogenesis in the central nervous system in both Xenopus laevis and the mouse, but their precise roles have remained unclear. We have characterised two family members in the chick, and have performed a functional analysis by gain- and loss-of-function experiments. This study revealed several specific roles for Ebf genes in the spinal cord and hindbrain regions of higher vertebrates, and enabled their precise positioning along the neurogenic cascade.

During neurogenesis, cell cycle exit appears to be tightly coupled to migration to the mantle layer and to neuronal differentiation. We show that antagonizing Ebf gene activity allows the uncoupling of these processes. Ebf gene function is necessary to initiate neuronal differentiation and migration toward the mantle layer in neuroepithelial progenitors, but it is not required for cell cycle exit. Ebf genes therefore appear to be master controllers of neuronal differentiation and migration, coupling them to cell cycle exit and earlier steps of neurogenesis.

Mutual activation between proneural and Ebf genes suggests that besides their involvement in the engagement of differentiation, Ebf genes may also participate in the stabilisation of the committed state. Finally, gain-of-function data raise the possibility that, in addition to these general roles, Ebf genes may be involved in neuronal subtype specification in particular regions of the CNS.


This article has been cited by other articles:


Home page
 GENES CELLSHome page
Y. Hattori, K. Sugimura, and T. Uemura
Selective expression of Knot/Collier, a transcriptional regulator of the EBF/Olf-1 family, endows the Drosophila sensory system with neuronal class-specific elaborated dendritic patterns
Genes Cells, September 1, 2007; 12(9): 1011 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lagergren, R. Mansson, J. Zetterblad, E. Smith, B. Basta, D. Bryder, P. Akerblad, and M. Sigvardsson
The Cxcl12, Periostin, and Ccl9 Genes Are Direct Targets for Early B-cell Factor in OP-9 Stroma Cells
J. Biol. Chem., May 11, 2007; 282(19): 14454 - 14462.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. A. Alcaraz, D. A. Gold, E. Raponi, P. M. Gent, D. Concepcion, and B. A. Hamilton
Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation
PNAS, December 19, 2006; 103(51): 19424 - 19429.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Bergsland, M. Werme, M. Malewicz, T. Perlmann, and J. Muhr
The establishment of neuronal properties is controlled by Sox4 and Sox11
Genes & Dev., December 15, 2006; 20(24): 3475 - 3486.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Warming, R. A. Rachel, N. A. Jenkins, and N. G. Copeland
Zfp423 is required for normal cerebellar development.
Mol. Cell. Biol., September 1, 2006; 26(18): 6913 - 6922.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. Croci, S.-H. Chung, G. Masserdotti, S. Gianola, A. Bizzoca, G. Gennarini, A. Corradi, F. Rossi, R. Hawkes, and G. G. Consalez
A key role for the HLH transcription factor EBF2COE2,O/E-3 in Purkinje neuron migration and cerebellar cortical topography
Development, July 15, 2006; 133(14): 2719 - 2729.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Taglialatela, J. M. Soria, V. Caironi, A. Moiana, and S. Bertuzzi
Compromised generation of GABAergic interneurons in the brains of Vax1-/- mice
Development, September 1, 2004; 131(17): 4239 - 4249.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y.-Q. Ding, J. Yin, A. Kania, Z.-Q. Zhao, R. L. Johnson, and Z.-F. Chen
Lmx1b controls the differentiation and migration of the superficial dorsal horn neurons of the spinal cord
Development, August 1, 2004; 131(15): 3693 - 3703.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003